Biomaterials with good biocompatibility and anti-bacterial property were becoming attractive to researchers, so we used the chemical method to produce anti-bacterial vacuum plasma sprayed titanium coatings and studied In vitro bioactivity, cytotoxicity and blood compatibility of the anti-bacterial coatings in this paper. In order to evaluate the bioactivity of the treated titanium coatings, the coatings were immersed in simulated body fluid (SBF). The treated titanium coatings showed good bioactivity in this experiment. Two different methods were used to assess the cytocompatibility of the treated titanium coatings. One was extract test; the other was direct contact test. The results indicated that cells spread and adhered well on the coatings. The blood compatibility of the coatings was evaluated by haemolysis ratios. The hemolysis ratios of the coatings were below 2%, indicating of nonhemolysis for the coatings.