The absorption of Cu2+ and Pb2+ in the heavy metal wastewater by distillers' grains can not only solve the problem of environmental pollution, but also change distillers' grains waste into resource. Structural characteristics of distillers' grains and its adsorption behaviors for the heavy metal ions of Cu2+ and Pb2+ were studied and effects of various parameters including pH, temperature, distillers' grains dose, initial Cu2+ and Pb2+ concentration and absorbed time on the absorption of Pb2+ and Cu2+ were evaluated. Sorption isotherms were also investigated. Results show that the rough surface, loose internal structure and hydroxy and amide groups of the distillers' grains are beneficial to the adsorption of Cu2+ and Pb2+ in the wastewater. The removal rate of the distillers' grains for Pb2+ (20.00 mg L−1) and Cu2+ (20.00 mg L−1) are, respectively, 96.72% and 87.70% under optimized conditions. The equilibrium sorption data are well demonstrated by Langmuir model.