The nose roundness is one of the most important indexes of lapping quality of rounded diamond cutting tools. Due to its special characters and requirements, a new measuring method was proposed based on atomic force microscope (AFM) and precision-revolution spindle. However, in the measurement the alignment errors of tools with the spindle axis have great influence on the measuring result. By a carefully deducting, the relations between the deviation and the alignment errors were presented. The deviation increases with the eccentric linearly, and has the same value with the eccentric. But it varies with θ dramatically and forms a parabola curve when the deflection angle is negative, otherwise, varies with θ gently and forms a flat Gaussian curve.