Paper Title:
Trapping Single Cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip
  Abstract

AC electrokinetics is one of many methods used to move particles in microfluidic channels. This paper presents single cell trapping efficacy using dielectrophoresis (DEP) force of two biochip designs; a planar biochip and the new sandwiched-insulation with back contact (SIBC) biochip. The new biochip, is structured on a glass slide, consists of microelectrode arrays patterned on top of Nickel-Chromium (NiCr) and Gold (Au) layers. Prior to the microelectrode patterning, a back contact layer of NiCr and Au was coated with SU-8 2005. Then, the SU-8 2005 or the insulation layer was patterned with arrays of microcavities. In contrast, the planar biochip consists of 2 layers; an SU-8 2005 insulation layer and NiCr and Au metal layers constructed on a Silicon Nitride (Si3N4) substrate. The electric field intensity results simulated using Comsol v3.5a software indicated that DEP force generated from the SIBC biochip are greater than the planar biochip design. Results from experiment with polystyrene microbeads and Ishikawa cancer cells also showed that the SIBC biochip has higher trapping efficiency than the planar biochip. These promising results indicate that the SIBC biochip is capable of trapping single cells and can be used to facilitate studies on intracellular activities using surface the replicating technique known as the Bioimprint technique.

  Info
Periodical
Edited by
B. J. Ruck and T. Kemmitt
Pages
188-194
DOI
10.4028/www.scientific.net/MSF.700.188
Citation
S. N. Ibrahim, L. Murray, J. J. Evans, M. M. Alkaisi, "Trapping Single Cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip", Materials Science Forum, Vol. 700, pp. 188-194, 2012
Online since
September 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Yong Jun Ko, Chul Ho Cho, Joon Ho Maeng, Byung Chul Lee, Yoo Min Ahn, Nahm Gyoo Cho, Seoung Hwan Lee, Seung Yong Hwang
Abstract:This paper presents a microbiochip which can detect an antigen-antibody reaction through an electrical signal in real time with high...
839
Authors: Chin Lung Chang, Weij Hong Ju, Ching Liang Liou, Jik Chang Leong, Lung Ming Fu
Abstract:This paper uses a femtosecond laser scriber to perform the direct-writing ablation of glass substrate for the development of microfluidic...
359
Authors: Ha Nguyen, Claudia Rauch, Martin Brandl
Abstract:A biochip for building up self assembled ferromagnetic structures was designed. The magnetic structures are self assembling onto magnetic...
95
Authors: M. Wesam Al-Mufti, U. Hashim, Tijjani Adam
Abstract:Currently, Computer simulation has become an essential part of nanotechnology and engineering and science. Digital analysis of components, in...
206
Authors: Siti Noorjannah Ibrahim, Maan M. Alkaisi
Chapter 9: Details and Machine Components, Mechatronics and Automation
Abstract:Microelectrode geometry has significant influence on particles trapping techniques used on bioanalysis platforms. In this paper, the particle...
543