Paper Title:
Gold Nanoflowers and Gold Nanospheres as Labels in Lateral Flow Immunoassay of Procalcitonin
  Abstract

In this study, new types of nanogold labels for lateral flow immunoassay of model antigen procalcitonin based on photometric and surface-enhanced Raman scattering methods of detection were obtained. The linear range of procalcitonin determination applying gold nanoflowers as a label was between 0.5 and 10 ng/mL. The limit of photometric detection was achieved at 0.1 ng/mL that was five times lower than the sensitivity of traditional lateral flow immunoassay with spherical gold nanoparticles as a label. In addition, the conjugate of polyclonal antibodies against procalcitonin with spherical gold nanoparticles labeled with 4-mercaptobenzoic acid as Raman reporter molecule was prepared and used as an immunoprobe in lateral flow immunoassay based on surface-enhanced Raman spectroscopy to improve assay sensitivity.

  Info
Periodical
Chapter
Chapter 1: Nanomedicine and Nanopharmacology
Edited by
Alexander Gusev, Tatiana Dyatcheck and Anna Godymchuk
Pages
47-53
DOI
10.4028/www.scientific.net/NHC.13.47
Citation
K. V. Serebrennikova, J. V. Samsonova, A. P. Osipov, D. Senapati, D. V. Kuznetsov, "Gold Nanoflowers and Gold Nanospheres as Labels in Lateral Flow Immunoassay of Procalcitonin", Nano Hybrids and Composites, Vol. 13, pp. 47-53, 2017
Online since
January 2017
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$35.00
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