This paper proposes a novel microfluidic system for cell/microparticle recognition and manipulation utilizing digital image processing technique (DIP) and optical tweezer under microfluidic configuration. Digital image processing technique is used to count and recognize the cell/particle samples and then sends a control signal to generate a laser pulse to manipulate the target cell/particle optically. The optical tweezer system is capable of catching, moving and switching the target cells at the downstream of the microchannel. The trapping force of the optical tweezer is also demonstrated utilizing Stocks-drag method and electroosmotic flow. The proposed system provides a simple but high-performance solution for microparticle manipulation in a microfluidic device.