The electrospinning technology has been used to fabricate organic nanofibers. As the nanofibers are aligned parallel and crossed, unique electrical and photonic properties are generated. Hereby, a frame of copper thread with a diameter of 0.8 mm was used to collect and align polymer nanofibers. SEM results showed that the nanofibers were parallel aligned between two copper threads. The crossed nanofibers arrays were obtained by layer-by-layer assembling on the parallel nanofibers. The influence factors, such as distance between two copper threads and collection time etc, were investigated.