Paper Title:
The Selective Protein Separations with Polyaminofunctionality on Controlled Silica Coating-Layers of Magnetic Nanoparticles
  Abstract

This work reported the development of the high throughput protein separation process with molecularly assembled silica-coated magnetic nanoparticles as a function of amino group numbers such as mono-, di-, and tri-aminofunctionality, in which the range of silica coating thicknesses were optimized to be interacted with protein. The protein separation efficiency was demonstrated as a function of each aminofunctional group and the particle sizes of the silica coated magnetic nanoparticles. The particles were prepared by the chemical precipitation of Fe2+ and Fe3+ salts with a molar ratio of 1:2 under basic solution. The silica coated magnetic nanoparticles were directly produced by the sol-gel reaction of a tetraethyl orthosilicate (TEOS) precursor, in which the coating layer serves as a biocompatible and versatile group for further biomolecular functionalization. To effectively capture the proteins, silica coated magnetic nanoparticles need to be functionalized reproducibly on the silica surface, and three kinds of amino functional groups were adapted as a function of number of amine using the mono-, di-, and tri-aminopropylalkoxysilanes.

  Info
Periodical
Solid State Phenomena (Volumes 124-126)
Edited by
Byung Tae Ahn, Hyeongtag Jeon, Bo Young Hur, Kibae Kim and Jong Wan Park
Pages
903-906
DOI
10.4028/www.scientific.net/SSP.124-126.903
Citation
M. E. Park, K. H. Kang, K. J. Kim, J. H. Chang, "The Selective Protein Separations with Polyaminofunctionality on Controlled Silica Coating-Layers of Magnetic Nanoparticles", Solid State Phenomena, Vols. 124-126, pp. 903-906, 2007
Online since
June 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Xu Gu, Jin Long Jiang, Dong Li
Abstract:Attapulgite clay coated Ag/AgBr/TiO2 visible light photocatalyst was prepared by an impregnation-deposition-precipitation method. The...
285
Authors: Xi Li, Jian Zhu, Fu Jian Lv, Jin Guo Wang, Zong Li Xie, Manh Hoang, He Xing Li
Chapter 7: Pollution Control Project (Air, Water, Solid)
Abstract:Two kinds of SnS2 materials were prepared through the hydrothermal route, respectively using thioacetamide (TAA) and thiourea as...
1708
Authors: Tao Hu, Yong Yang, Jian Wen Xia
Chapter 3: Material Engineering and Science
Abstract:Modification of attapulgite by KH-570 for formaldehyde adsorption and characterization of the resulting sorbents by SEM, BET, FT-IR and...
3495
Authors: Zhi Hua Pang, Xiao Shan Jia, Kai Liu, Zhen Xing Wang, Qi Jing Luo, Jun Luo
Chapter 1: Environment Materials and Chemical Environment
Abstract:Taking the organic modified montmorillonite as a carrier and dispersant, the supported nanoscale zero-valent iron materials with different...
155
Authors: Yan Jie Bi, Wen Yan Li, Chen Yuan, Bin Song Wang
Chapter 1: Environmental Chemistry, Chemical Manufacturing, Technologies and Engineering
Abstract:In this paper, molecularly imprinted polymers with high selectivity for nonylphenol (NP) were synthesized by sol-gel process using...
55