Papers by Author: Bin Du

Abstract: An electroanalysis method of determining Chloramphenicol (CAP) based on the eletrocalysis property of DNA-hydroxyapatite modified electrode (dsDNA/HAp/GCE) was setted up. In KH₂PO₄-Na₂HPO4 buffer solution with pH 6.0 cotaining 0.1 mol·L^-1 KCl, the peak current was proportional to the concentration of CAP in the range of 2.80 × 10^-7 to 3.60 × 10^-6 mol·L^-1, with a detection limit of 1.35 × 10^-7 mol·L^-1. The linear regress equation was i_p (μA) = 5.865 + 0.4784 c (µmol·L^-1), and the linear correlation coffcient was 0.9942. The method has been successfully applied to the quantitative analysis of CAP in real Chloramphenicol eye drops samples, which totally meet the demand of microanalysis. The relative standard deviation samples analysis results was 1ess than 3%.

Abstract: A review is provided dealing with the definitions and classifications of biosensors, as well as their basic working principles. Then, their applications to the environmental monitoring, which include the monitor of biochemical oxygen demand (BOD), bacterial count and various organic and inorganic pollutants, e.g. nitrogen compounds, heavy metal ions, organophosphorus pesticides, phenolic compounds, toxic compounds, carcinogens, teratogens, benzenes, sulfur dioxide, aldehydes and ethanols are presented. In the end, some suggestions were given with respect to the current problems of biosensors and the future applications of biosensors were also discussed.

Abstract: It was the first time to study the catalytic effect of mesoporous magnetic Fe₃O₄ nanoparticles on the biohydrogen production. The mixed culture used in this study just suffered from an alkaline shock and lost its bioactivity of hydrogen production. We use mesoporous Fe₃O₄ nanoparticles and ferrous ions as activators to recover the bioactivity of the mixed culture. The results indicate that the improvement of biohydrogen yield by mesoporous Fe₃O₄ was obvious larger than that by ferrous ions. The maximum yield of cumulative hydrogen production was obtained at the mesoporous Fe₃O₄ nanoparticles of 400 mg·L^-1, which is 26% higher than that of the blank. The lag phases for hydrogen production in the tests added with mesoporous Fe₃O₄ nanoparticles were decreased to 12 h, which are 50 h less than those of the corresponding ferrous ions and blank tests.