Papers by Author: Chun Guang Wang

Abstract: Plasmid DNA was extracted from all 38 strains of chicken Escherichia coli by alkaline lysis method. The agarose gel electrophoresis showed 23 plasmid patterns. The strains from the same source had the same, similar, or quite different plasmid profiles, while those from different sources generally did not have the same plasmid profiles. Therefore, the plasmid profiles can provide basic materials for epidemiological investigation on chicken Escherichia coli at molecular level.

Abstract: Thirty-eight isolates of Escherichia coli (E.coli) were isolated from chickens either ill or dead suspected with E.coli. These chickens come from chicken breeding farms in the districts of Baoding, Qinhuangdao and Beijing of China. The results of the antibiotics sensitivity test in the thirty-eight isolates to twenty-four kinds of antibiotics show that all isolates have presented antibiotic resistance disparately as well as multiresistance. All isolates are sensitive to Cephalosporins antibiotics and Aminoglycosides antibiotics such as Amikacin, Cefazolin, Cefradine, Cefoperazone and Ceftriaxone. Thirty-eight isolates are extremely resistant to Penicillins antibiotics, Lincomycins antibiotics, Tetracyclines antibiotics and Macrolides antibiotics such as Midecamycine, Lincomycin, Carbenicillin, Tetracycline, Amoxicillin. Antibiotics sensitivity test provides a guidance for antibiotic application and scientific research on poultry farms.

Abstract: An Ag-Ce-TiO2 composite film was prepared by incorporating Ag in the Ce-TiO2 films with an impregnation method through photoreduction. The bactericidal activity of the Ag-Ce-TiO2 composite film and the effect of deposition of silver and doping cerium in the different irradiations on the antibacterial performance were assessed against Escherichia coli (E. coli). TIhrough characterization of X-ray diffraction (XRD), X-ray photoelectronic spectra (XPS) and UV/VIS diffusive reflectance spectra (DRS), it was found that the Ag-Ce-TiO2 composite film was composed of predominant anatase titania, cerium oxides (in the form of Ce2O3/CeO2) and silver nanoparticles. Moreover, the bactericidal activity of the Ag-Ce-TiO2 composite film and the effect of deposition of silver and doping cerium in the different irradiations on the antibacterial performance were assessed against Escherichia coli (E. coli). The results show that the Ag-Ce-TiO2 composite film has much higher antibacterial efficiency than that of glass and pure TiO2 film either in the room light or in the dark. Therefore, this composite film is promising in applications of antimicrobial and self-clean technologies.