Papers by Author: Dong Min Liu

Abstract: The energy method was used to analysis the deformation of acrylic waterproof membrane under the uniform load and to calculate the maximum central deflection according to the clamped circular plate. Because the shape of the deformation of waterproof membrane bulge was similar to semi-ellipsoid, the curve length corresponding to the maximum central deflection was calculated by elliptic equation. According to the curve length, the deformation could be calculated under the different water pressure and membrane thickness. Comparing the deformation with the maximum elongation ratio, the water pressure and the thickness of waterproof membrane which ensure the safety could be calculated in theory. If the water pressure was known, the thickness of spray-membrane could be determined in the tunnel engineering.

Abstract: In this study, we mixed peeled and grinded Juglans mandshurica nuts with milk, using Lactobacillus casei and Streptococcus thermophilus as culture, the ration is 1:1. After fermented, we obtained enriched Juglans mandshurica nut yoghurt. The optimal formula of the yoghurt was determined by the L9 (34) orthogonal test. The best formular was: solid-liquid ratio 1:6, pulp-milk ratio 2:8, and filtered with an 80 mesh sieve. This dairy product contains the nutrients from Juglans mandshurica nuts, fresh milk and probiotics; tastes sour and sweet with a walnut fragrance; and is a kind of functional food with a great economic value and market potential for development.

Abstract: The energy method was used to analysis the flexural property of acrylate waterproof membrane in local loads during the period of construction and service. The membrane was formed by spraying-membrane waterproof technique for tunnel engineering. The maximum central deflection of the clamped circular plate was calculated in the uniformly distributed and concentrated loads. The minimum safety thickness was calculated in theory during the course of using. It was expected to provide theoretical reference for the further improving of spraying-membrane waterproof technique.

Abstract: The present study was carried out to determine the effect of latrunculin A (Lat-A) and FBS on the developmental potentials of electrically activated porcine oocytes matured in vitro. Electrically activated MII oocytes were incubated for 2 hr in mPZM-3 supplemented either with 0、20 nM、1 μM Lat-A or 2.2 μg/ml cytochalasin B (CB). They were then removed and placed in mPZM-3 with or without 10% FBS (addition) on Day 4 for further development. The nuclear activation rate was significantly improved when activated oocytes were cultured in mPZM-3 with Lat-A (P<0.05). At higher dosage (1μM) of Lat-A, the extrusion of second polar body was inhibited while the cleavage rate increased significantly (P<0.05). Significant interaction between Lat-A and FBS supplementation on Day 4 was observed during in vitro development (P<0.05). The hatched blastocyst rates and cell numbers per blastocyst were significantly enhanced with the supplementation of FBS (P<0.05). The blastocyst formation, hatched blastocyst rates and mean cell numbers per blastocyst of 1 μM Lat-A treatment with FBS supplementation were significantly higher than those of 20 nM Lat-A and CB treatments with FBS supplementation (P<0.05), but the individual effect of FBS supplementation was not observed on blastocyst formation in 20 nM and 1 μM Lat-A treatments. While significant difference was seen in blastocyst formation and cell numbers per blastocyst among all the treatments without FBS supplementation (P<0.05). These results indicate that Lat-A especially at the dosage of 1 μM can significantly improve the developmental potential of electrically activated porcine oocytes, and the FBS supplementation on Day 4 of development have an additional effect on porcine embryo development.