Papers by Author: Feng Huei Lin

Abstract: DP-bioglass is one of biodegradable glasses, which can be used as bioactive material in soft tissue and bone. It was often used in orthopedy and plastic surgery. Recently, bioglass was also used as a carrier for drug and gene delivery systems. Additionally, ferrimagnetic DP-bioglass can be potential candidates for magnetic induction hyperthermia, by using a magnetic field. The aim of this work is the preparation and characterization of surface-modified ferrimagnetic DP-bioglass. First DP-bioglass has to be surface-modified with polyethylene glycol (PEG) and folic acid (FA) to improve its intracellular uptake and ability to target specific cells. PEG-FA complex was synthesized using carbodiimide (DCC) to link PEG with FA. Then PEG-FA complex were immobilized on the surface of DP-bioglass by using amino-silane (AEAPS) as a coupling agent. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H NMR), and thermogravimetric analysis (TGA) was used to demonstrate this immobilization process. In biological study showed that immobilized ferromagnetic DP-bioglass with PEG-FA was non-cytotoxicity and significantly enhanced the intracellular uptake of DP-bioglass by target cells.

Abstract: Three-dimensional gelatin-chondroitin 6 sulphate-hyanuronic acid biomatrix was used as the scaffold to investigate the phenotypic and molecular expression in human keratinocytes (K) and dermal fibroblasts (FB) in three different culture conditions in vitro. The cells were cultured in either monolayer (K or FB only) or coculture (K&FB) model. The deposition of basement membrane proteins secreted by these two kinds of cells was quantitatively characterized by real-time PCR. In the results, dermal fibroblasts were shown to synthesize and deposit laminin 5, type IV and type VII collagen, whereas keratinocytes produced integrin alpha 6 and beta 4 as well as laminin 5 and collagen type IV, VII. Interestingly, the integrin beta 4 subunit was not expressed either in keratinocytes or dermal fibroblasts monoculture but was seen in organotypic coculture model in the early culture period. Furthermore, we found that the expression of those marker compounds was reciprocally regulated when keratinocytes and dermal fibroblasts were cultured together. These results indicated that keratinocyes and dermal fibroblasts worked together to reconstruct dermal-epidermal basement membrane (BM) zone. In brief, our data provide the first time in directly quantifying the expression of BM proteins by using real-time PCR, and also demonstrate that BM proteins were regulated by cell-cell interaction.