Papers by Author: Gang Wang

Abstract: Stimuli responsive electrospun fibers are obtained considerable attention as excellent functions and properties. An overview is given on recent advances made in the development from component to structure of electrospun fiber and applications in the aspects of physical and chemical stimuli. The specific features for these electrospun fibers are highlighted and applications are discussed as regeneration medical engineering and drug-releasing implants materials. Furthermore, the development of multi-component and multi-stimuli functions electrospun fiber is proposed in the future perspective

Abstract: Highly ordered mesoporous silica nanofibers with no core/sheath structure were prepared by coaxial electrospinning combined with the solvent evaporation induced surfactant assembly process. The mesoporous structures have been proved and the effect of different cosurfactants on pore sizes of mesoporous silica nanofibers has been investigated.

Abstract: Numerical simulation of titanium alloy powder during hot isostatic pressing (HIP) with Shima model is studied in this paper. Material parameters in this model were determined based on uniaxial compression test with different relative density specimens. A titanium alloy ring sample was designed and formed by hot isostatic pressing. The sample was analyzed by using Shima model in the platform of MSC.Marc with the determined parameters of titanium alloy (Ti-6Al-4V). The results of simulation with Shima model were in a good agreement with that result of experiment. Finally, the model is successfully applied to complex model of impeller to predict the deformation during HIP.

Abstract: This study investigated the utility of genetically modified cell line for fast and non-destructive cytotoxicity evaluation of biomaterials. The EGFP(enhanced green fluorescence protein)-expressing plasmid pcDNA-EGFP was constructed, and electroporated into ROS 17/28 osteoblastic cells to generate an EGFP-labeled stable cell line, ROS-EGFP. This genetically modified cell line provided two unique opportunities to qualitative and semi-quantitative evaluation of cell growth on biomaterials without destruction of samples. Firstly, utilizing the fluorescence of EGFP expressed in the cells, the viability state of cells on biomaterials was evaluated using a fluorescent light microscope. Secondly, the proliferation of cells on biomaterials, which was identified by MTT assay，was demonstrated according to the microscopically counted fluorescent cell numbers. From the results, it could be concluded that the ROS-EGFP cell line was an effective tool to trace the fate of cells on biomaterials and to evaluate the biocompatibility of biomaterials to cell growth in vitro.

Abstract: The mRNA expression of Cbfa1 and osteocalcin gene induced by calcium phosphate ceramics (Ca/P) were quantitative analyzed according to real-time RT-PCR method in this work. C2C12 cells were co-culture with four kinds of porous Ca/P ceramics for 2 and 5d without adding other growth factors. The four kinds of Ca/P ceramics were pure hydroxyapatite (HA) sintered at 1250°C and HA/TCP with a ratio of 60/40 sintered at 1100°C (HT1), 1200°C (HT2) and 1250°C (HT3) respectively. Real-time RT-PCR analysis found the Ca/P ceramics induced positive expression of Cbfa1 and osteocalcin in C2C12 cells, After 5 days culture, Cbfa1 and osteocalcin showed obvious higher expression compared with that in 2 days. Cbfa1 and osteocalcin expression in BCP was much higher than HA, and the expression level of osteocalcin was HT1>HT2>HT3>HA. Our results showed that Ca/P ceramics alone were sufficient to induce C2C12 cells to osteoblastic differentiation and the sinter temperature and phase composition of Ca/P ceramics could affect their osteoindctive capacity significantly.