Papers by Author: Guo Ying Zhou

Abstract: Plant extracts were efficient, safe bio-control stuff. In this paper we used two kinds of plant extracts, Eugenol and Baicalein, to against Colletotrichum gloeosporioides and Agaricodochium camellia by growth rate method. The results show that the inhibition effects of eugenol and baicalein were increasing as the rising concentration form 0 to 200ug/ml. The EC₅₀ of eugenol against Colletotrichum gloeosporioides and Agaricodochium camellia were 30.37ug/ml and 51.55ug/ml respectively, the EC₅₀ of baicalein against Colletotrichum gloeosporioides and Agaricodochium camellia were 61.26ug/ml and 49.83ug/ml respectively. The synergistic interaction values of the compounded agents with different volume ratio against Colletotrichum gloeosporioides and Agaricodochium camellia were between 0.5 and 1.5. When the volume ratio of eugenol and baicalein were 1:1 ,the prevention and treatment effects of compounded agent and carbendazim with were the same, up to 60%.

Abstract: Glyceraldehyde 3-phosphate dehydrogenase is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In the present study, some characters of the amino acid sequence of GAPDH of L.deliciosus were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of GAPDH was 7.08 and the theoretical molecular weight of GAPDH was 26165.9 Da; the total number of atoms was 3714. It was a stable protein. There were 7 glycosylation sites and it was a tetrameric NAD-binding enzyme involved in glycolysis and glyconeogenesis. N-terminal domain is a Rossmann NAD (P) binding fold. C-terminal domain is a mixed alpha/antiparallel beta fold.

Abstract: The pine is an important tree species in China, while the pine wilt disease is a devastating disease of pine trees. Pine wood nematode (Bursaphelechus xylophilus Steiner & Buhere, 1934 Nickle, 1981) is a world recognized major alien species in the world. It’s from the United States, but pine wood nematode does not seriously endanger the pine in China; the most endangered place is Japan, after several decades of research and control, it has been basically able to control the occurrence of pine wilt disease. The spread trend of pine wilt disease in China grows more and more obvious, which has become an important intrusion pathogenic organism. It not only causes a devastating threats the million hectares pine forest in southern China, but also affects China’s economy and social sustainable development, what’s more, it will damage some of famous scenic spots and cultural heritage sites in China. Meanwhile, the pine wood nematode is a technical barrier, which seriously impacts on China’s import and export trade. The pathogen, pathogenesis, modes of transmission and means of distribution patterns, rapid detection and control methods of pine wilt disease are reviewed in this paper, and we hope that it can provide references for effective control of pine wilt disease in China.

Abstract: Camellia anthrax disease is the main disease in producing areas of China. It occurred widespread, caused fruit and flowers dropped seriously, resulting in huge economic losses. In this paper, we separated pathogen with organizational separation method from the diseased leaves and fruit which were infected by Camellia anthrax. Then we proved, by Koch rule, it was pathogen which caused Camellia anthrax disease. According to their morphological characteristics, we identified that it was Colletotrichum gloeosporioides. From the Camellia healthy leaves, we isolated totally 125 strains endophytic fungi. Through the primary screening, 13 strains were detected had inhibited on Colletotrichum gloeosporioides. After the secondary screening, we obtained three strong antagonistic fungi, numbered Cd7, Sy3, Hy19, the inhibition rates separately were 60.3%, 59.7%, 57.8% on the PAD flat.

Abstract: Using growth rate method, biological activities of 20 water extracts from 20 different plants were assayed against Colletotrichum gloeosporioides and Agaricodochium camellia Liu. The results show the inhibition rates of the extracts of Syzygium aromaticum, Scutellaria altissima L, Aloe Vera, Lonicera japonica Thunb, Andrgraphis paniculata are more than 60%. The best inhibition effects was S. aromaticum, respectively 96.2% and 98.2%. The toxicity test indicates that the EC50 of S. aromaticum and S. altissima L extracts and the mixture at the 1:1, 1:2, 1:5, 2:1, 5:1 volume ratio against C. gloeosporioides and A. camellia are, respectively ,4.2 and 3.68mg/ml,4.52 and 6.90 mg/ml,5.28 and 5.33 mg/ml,5.84 and 7.01 mg/ml,4.89 and7.01 mg/ml,5.24 and 6.11 mg/ml,4.77 and 5.65mg/ml. Synergistic interaction values are >0.5 and <1.5.

Abstract: Bamboo fiber is a new natural green environmental protection fiber material with good characteristics of good permeability, unique elasticity, transient absorption, antibacterial, odor resistance and stronger vertical and horizontal intensity. Bamboo pretreatment can improve the biological degumming efficiency of bamboo lignin, reduce microbial degumming time, and reduce production costs. The results show that the removal efficiency of pectin, hemicelluloses and lignin are better after the pretreatment of bamboo mercerization. Through orthogonal experiments, it can be got the optimum combination of bamboo fiber alkali cooking process is A2-B2-C2-D1, namely: concentration of NaOH is 6.0g / L, its time is 60min, temperature 100 °C, and bath ratio 1:20. In these four factors, the greatest impact on the removal of lignin is temperature, followed by is degumming time, then the concentration of alkali, and the bath ratio is the smallest. The best conditions of bamboo fiber bleaching is: hydrogen peroxide 25 ~ 35g / L, temperature 30 ~ 40 °C, treatment 45 ~ 60min. The process of the pretreatment of bamboo is: clip → Roll → high-pressure steaming → roll→ immersion separation → alkali leaching treatment→ scouring → rolling combing →washing→ drying →crude fiber → bleaching.

Abstract: The natural bamboo fiber is a green environmental protection type fiber material extracted from bamboo. Compared with other fibers, it has better permeability, unique resilience, transient water absorption, antibacterial activity, deodorant activity, and a strong lateral and longitudinal strength. This paper reviews the structure of natural bamboo fiber and its characteristics, physical morphology, chemical composition, hygroscopicity and moisture liberation, antibacterial activity, anti-ultraviolet function, spinning performance of the development and utilization status of bamboo fiber, which provides reference for the research and utilization of natural bamboo fiber.

Abstract: Rhizopus oryzae is the most common causative agent of zygomycosis and has a world-wide distribution with a high prevalence in tropical and subtropical regions. Chitin deacetylase acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. In the present study, some characters of the amino acid sequence of Chitin deacetylase of R.oryzae were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of Chitin deacetylase was 5.03 and the theoretical molecular weight of Chitin deacetylase was 49177.9 Da; total number of atoms was 6786; the extinction coefficient was 108665(280 nm).The N-terminal of the sequence considered was M (Met) and the estimated half-life was 30 hours. The instability index (II) was computed to be 32.67. Aliphatic index was 67.91. There were 20 glycosylation sites,a signal peptide and conserved domains. It was a hydrolase.

Abstract: Gibberella moniliformis is a common disease in C. oleifera, and always results in significant economic losses. Phosphoenolpyruvate carboxykinase is a critical gluconeogenic enzyme and catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. According to the relative conservation of homologous gene, a bioinformatics strategy was applied to clone phosphoenolpyruvate carboxykinase gene of Gibberella moniliformis by blasting search of EST database with homologous gene cDNA of Neurospora crassa and identified. Some characters of the PEPCK that can encode amino acid were analyzed and predicted by the tools of bioinformatics in the following aspects, including the composition of amino acid sequences, physical and chemical, O-glycosylation site and tertiary structure of protein and function. These results showed that the full-length of PEPCK was 1962 bp and it contained a complete ORF (1671bp), encoded 556 amino acids, which are much conserved in ascomycetes. The calculated molecular weight of PEPCK was 61893.1 Da, theoretical pI of 5.77, 20 a-helix, 37 sheets, 7 glycosylation sites; it was a stable protein with active site ATP -binding site, metal-binding site and substrate-binding site.

Abstract: Pycnoporus cinnabarinus is a plant pathogen. It is common in many areas and is widely distributed throughout the world. Laccases of are some of the few oxidoreductases commercialized as industrial catalysts. In the present study, some characters of the amino acid sequence of P.cinnabarinus laccase (Lac1) were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of manganese peroxidase was 4.81 and the theoretical molecular weight of manganese peroxidase was 56292.0 Da; total number of atoms was 7806; the extinction coefficient was 58120 (280 nm). The N-terminal of the sequence considered was M (Met) and the estimated half-life was 30 hours (mammalian reticulocytes, in vitro). The instability index (II) was computed to be 34.50; this classifies the protein as stable. Aliphatic index was 82.64. Grand average of hydropathicity (GRAVY) was -0.063. There were 8 glycosylation sites, a signal peptide and conserved domains.