Papers by Author: Ho Hyun Song

Abstract: In this study, novel polycaprolactone/hydroxyapatite (PCL/HA) scaffolds were prepared to increase mechanical properties and degradation of PCL/HA ones for bone tissue engineering. PCL macromers were synthesized through the reaction of PCL diol (Mn: 530, 1250, and 2000) and PCL triol (Mn: 900) with acryloyl chloride and confirmed using nuclear magnetic resonance spectrometer (NMR) and fourier transform infrared (FTIR). The PCL/HA scaffolds were prepared by cross-linking of PCL macromer in the presence of HA by UV treatment and freeze drying methods. Mechanical property and porosity as well as degradability of the PCL/HA scaffolds were also investigated. PCL/HA scaffolds showed faster degradation and higher compressive modulus than those of PCL itself due to their low crystallinity and modification of terminal groups. The pore morphology and pore sizes of the PCL/HA scaffold were checked by scanning electron microscope (SEM). Cell cytotoxicity and proliferation of MG-63 osteoblast cultured onto the PCL/HA scaffold was assessed by lactate dehydrogenase (LDH) assay and Alamar blue assay, respectively. The novel PCL/HA scaffold appears to be suitable for bone substitutes.

Abstract: The purpose of this study is to make use of trans10,cis12 CLA (t-CLA) that has potential for proliferation and differentiation to form adipocyte on the collagen-coated surface. Results provided evidences of good adhesion, growth, viability, and differentiation of adipocyte on collagen-coated surface compared with non-coated surface. Also, the results showed that mouse 3T3-L1 preadipocyte can be successfully and reproducibly cultured on the collagen-coated surface, and the adipocyte precursor cells placed on the collagen-coated surface are able to undergo full maturation into adipocytes in the control cells. Moreover, glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 preadipocyte cultured on collagen-coated surface with t-CLA was higher than that on polystyrene (PS) surface due to higher cell adhesion and cell viability. These results suggest that collagen coating may provide a promising approach to develop a new adipocyte replacement strategy using CLA.