Papers by Author: Jiao Sun

Abstract: The present study was designed to investigate the effects of hydrolyzed fish collagen (HFC) on the cytokine production of lipopolysaccharide (LPS)-induced human umbilical vein endothelial cells (HUVECs). The in vitro inflammation model was established using LPS-induced HUVECs.The cell viability of HUVECs and the secretion level of inflammatory cytokines,IL-6, IL-8, and TNF-α from LPS-treated HUVECs exposed to HFC were determined respectively using MTT and ELISA assays. Our results indicate that HFC promoted HUVECs proliferation, and significantly reduced IL-6, IL-8, and TNF-α production in LPS-stimulated HUVECs. It suggested that HFC had a prominent anti-inflammatory property, HFC could be considered as a promising candidate for anti-inflammatory wound dressing.

Abstract: The aim of the present study was to evaluate the cell viability and angiogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) stimulated by a new fabricated nagelschmidititebioceramic. The Ca₇Si₂P₂O₁₆bioceramics (CSP) were extracted with L-DMEM for 72h. The extracts were analyzed byInductively Coupled Plasma optical emission Spectrometry (ICP-OES) to determine the elemental concentrations of calcium, siliconand phosphorus (Ca, Si and P). MTT assay was conducted to evaluate the cell viability of original extracts. Gene expression of endothelial specific markers including vWF and CD31 was assayed forBMSCs cultured in original extracts on day 3 and 10 to determine the angiogenesis.The results showed that the concentration of Ca, P and Si ions released from CSP ceramic were 112.0mg/L, 62.0mg/L and 19.8mg/L respectively, which were much more than β-TCP and control group. CSP also showed more significant cell viability and higher exression level of vWF and CD31 when rBMSCs were cultured with its extracts. This study indicated that this new bioceramics are promising bioactive materialsfor bone tissue regeneration.

Abstract: The aim of the present study is to evaluate the cupric ion releases and cytotoxicity of Cu-bearing intrauterine device (IUD) with different surface areas. TCu380, GCu300 and MCu165 were selected and extracted with MEM for 24h. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was employed to determine the concentration of Cu ions. MTT assay was conducted to evaluate the cell viability of original and diluted extracts. Oxidative stress indicators such as superoxide dismutase (SOD) and lipid peroxidation (MDA) were measured. The results showed the concentration of Cu ions released from three kinds of IUDs were 114.836mg/L, 91.161mg/L and 85.750mg/L, respectively. Cell viability of all original IUDs extracts was around 10%. TCu380 showed more significant cytotoxicity and higher oxidative stress level than other two IUDs when extracts were diluted. The study suggested that there was a positive correlation between the quantity of released Cu ions and cell damage effect in Cu-IUDs. The mechanism of cytotoxicity might be elevation of oxidative stress.

Abstract: Mesoporous bioactive glass (MBG) has superior bioactivity and degradation than non-mesoporous bioactive glass (BG) in vitro. But the biological effect of MBG in vivo is still unknown. In this study, MBG powders with 20μm were implanted into the femoral condyles in SD rats. BG powders with 20μm were used as a control. The local degradation and osteogenesis were observed at 1 week and 4 weeks after implantation, and the systemic toxicity of the degradation products were also evaluated simultaneously. The results revealed MBG powders had the faster rate of degradation and better osteogenesis effect than BG powders at 4 weeks, although the most of material still remained in situ. Histopathological analyses indicated the degradation products did not have any damage to major organs such as liver and kidney. In conclusion, this preliminary study demonstrated that MBG powders have more excellent biological effect at 4 weeks than that of BG in vivo. However the long-term effect needs to be confirmed.

Abstract: The present study was designed to investigate the effects of porcine collagen peptides on biological behavior of PC12 cells, the Amino acid composition of porcine collagen peptides was analyzed using Amino Acid Analyzer, the contact angles were measured with a goniometer, and after treatment with porcine collagen peptides, the impact of peptides on cells proliferation and differentiation was evaluated using MTT assay and Real-time PCR respectively. porcine collagen peptides is found to contain mainly Gly, Pro and Hyp, the contact angles of porcine collagen peptides surfaces were approximately 23.3°. MTT Results revealed that collagen peptides promoted PC12 cells proliferation at the given concentrations with its maximum effect at the concentration of 0.2 mg/ml (P<0.01), Real-time PCR Results revealed the poricine collagen peptides had no influence on the expression of specific neuronal marker β3-tubulin. Results of this study suggest that based on their bioactivity, porcine collagen peptides could be promising candidates for biomaterials.

Abstract: The aim of the present study is to evaluate the in vivo biological behaviour of porous β-CS/PDLGA scaffolds. The scaffolds were implanted in critical-sized femur defects ( 6 ×10 mm) for 4, 12 and 20 weeks with β-TCP scaffolds as the control. The in vivo bone regeneration of the scaffolds were investigated using sequential histological evaluations and Micro-CT technology. Results showed that the β-CS/PDLGA scaffolds could stimulate bone regeneration and degrade progressively at a rate proportionate with the regeneration of new bone as compared with β-TCP scaffolds. The present study suggested the potential application of β-CS/PDLGA scaffolds in hard tissue regeneration.

Abstract: Despite applications of Silica nanoparticles (SiO2 NPs) have been developed in the biomedicine field, relatively few studies have been conducted to determine the hepatotoxicity of SiO2 NPs. In the present study, rat hepatic cells (BRL cells) were directly exposed SiO2 NPs. The cytotoxicity of SiO2 NPs at different doses was studied by analyzing the cell viability, cell apoptosis, reactive oxygen species (ROS), glutathione (GSH), superoxide dismutase (SOD), and lipid peroxidation (MDA). The results showed that after treatment with SiO2 NPs for 24 h, the viability of BRL cells was reduced. In addition, SiO2 NPs were also found to induce cell apoptosis and oxidative stress. In summary, exposure to SiO2 NPs resulted in a dose-dependent cytotoxicity in BRL cells that was associated with increased oxidative stress.

Abstract: Insufficience of osteogenesis and antimicrobial effect have been still impacted the long term clinical success rate of dental implants. A nanostructured titanium surface prepared by hydrothermal treatment with H2O2 was evaluated on its osteoblastic viability and antibacterial effect. Samples were divided into 2 groups: untreated pure titanium surface (Ti) and a nanostructured titanium surface (NT). The antibacterial activities against S.mutans and C.albicans were measured by film applicator coating assay, as well as the live/dead bacteria stain. The osteoblastic viability was investigated by SEM and MTT assay. Results showed that the active microbia on NT was reduced at 24h (P<0.05) significantly according to the live/dead bacteria stain and film applicator coating assay, which could also enhance the osteoblast viability. Therefore, a nanostructured titanium surface exhibits good antibacterial activity on S.mutans and C.albicans, and promoting osteoblast viability, which will be a potential kind of dental implant material.

Abstract: Hydroxyapatite (HAP) and tricalcium phosphate (TCP) nanoparticles (NPs) are in current use covering a wide range of medical applications, hence, it is indispensable to study the potential side effect acting on living organism, and to figure out the biocompatibility evaluation model of nanoparticles for standardization and methodology. In the present study, HAP and TCP NPs were selected and their diameters were within 30-80nm. Cytotoxicity in vitro was analyzed by agar overlay and direct contact exposure test was conducted to evaluate potential toxicity to SD rat macrophages, and hemolysis test of NPs was performed in compliance with ISO 10993-12 guidelines. The results showed that both HAP and TCP NPs could inhibit proliferation of macrophages when their concentration was reached to 20 µg/ml, and the extent of haemolysis would be increased more than 5% while 1000µg/ml NPs were contacted with rabbit blood cells. Furthermore, TCP NPs could induce obvious hemolysis reaction and inhibit proliferation of macrophages compared with group of HAP NPs at the same concentration.

Abstract: Nano-sized silicon dioxide (SiO₂) is widely utilized in artificial bone, artificial tooth, interventional catheters and drug delivery system，so it is imperative to study SiO₂ NPs on the role of the body damage. Consulted with standard reference of GB/T16886, 20 and 80 nm SiO₂ NPs were selected to prepare suspension (1mg/ml) for subacute systemic toxicity; 2000mg/ml suspension for MTT; and extracts (0.1g/ml) for hemolytic test. The results show that the hemolysis rate are all more than 5%, after its continuous injection of 2W nanoparticles into SD rats and New Zealand Rabbits, the alterations on the hemoglobin, hematocrit, mean corpuscular volume and other indicators have gained in blood routine test, and there was a marked inhibition on the L929 cells of SiO₂ NPs. Therefore, these two kinds of particle size of SiO₂ NPs have certain extent of injury effect on the blood system, and the contact time of NPs in the blood compatibility evaluation has play a more important role than the others.