Papers by Author: P. Pripatnanont

Abstract: Platelet-rich fibrin (PRF) is platelet concentration that contains growth factors and acts as a biodegradable scaffold. The aims of this study were to determinethe quantity ofentrappedgrowth factors (Platelet derived growth factor BB, PDGF-BB) in the PRF and radiographically assess the stability of the fibrin in maintaining the lifted sinus space in minipigs. From the in vitro study, PRF was found slowly releasing thegrowth factor, PDGF-BB, during the ex-vivo period of 60 minutes, and the amount (1,963.93±380.17 pg/ml) was comparable to the total amount from immediate extraction either by physical (2,492.2±199.78 pg/ml) or chemical lysis (2,227.32±566.59 pg/ml).In vivo study, PRF wasable to be retained in the sinus of minipigs with minimal collapse during the first 2 weeks after application. PRF has been proven to be a source for growth factors and is able to be retained in the body during the initial period of wound healing.

Abstract: The biphasic calcium phosphate (BCP) concept was introduced to overcome disadvantages of single phase biomaterials. In this study, we prepared BCP from nanoHA and β-TCP that were synthesized via a solid state reaction. Three different ratios of pure BCP and collagen-based BCP scaffolds (%HA/%β-TCP; 30/70, 40/60 and 50/50) were produced using a polymeric sponge method. Physical and mechanical properties of all materials and scaffolds were investigated. XRD pattern proved the purity of each HA, β-TCP and BCP. SEM showed overall distribution of macropores (80-200 µm) with appropriate interconnected porosities. Total porosity of pure BCP (93% ± 2) was found to be higher than collagen-based BCP (85%± 3). It was observed that dimensional shrinkage of larger scaffold (39% ± 4) is lower than smaller one (42% ± 5) and scaffolds with higher HA (50%) ratio experienced greater shrinkage than those with higher β-TCP (70%) ratio (45% ±3 and 36% ±1 respectively). Mechanical properties of both groups tend to be very low and collagen coating had no influence on mechanical behavior. Further studies may improve the physical properties of these composite BCP.