Papers by Author: S. Rassameemasmaung

Abstract: 75:25 poly (DL-lactide-co-ε-caprolactone) electrospun resorbable membranes were prepared by electrospinning and film casting techniques. Primary cultures of human periodontal ligament (HPDL) fibroblasts cells were seeded on the electrospun membranes, film-cast membranes, and control glass cover slips. The number of cell attached was determined at 1, 3, 24 and 72 h after cell seeding. Cell proliferation on the membranes was determined by MTT assay at 1.5 h and 1, 3, 5 and 7 days after cell seeding. The morphology of cells was also determined by SEM. The results indicated that the cell attached number and cell proliferation on the electrospun membranes were significantly higher than that of the film-cast membranes at every time point. From an SEM study, HPDL cells could healthy attach on both, the electrospun membranes and the film-cast membranes. HPDL cells were firmly attached to the membrane matrix in the electrospun membrane group. In the film-cast group, HPDL were loosely attached to the membrane surfaces. In conclusion, an electrospun membrane had better ability to promote HPDL cell attachment and proliferation than did film-cast membranes.

Abstract: The aim of this study was to fabricate 75:25 poly(DL-lactide-co-e-caprolactone), poly(DLL-co-CL) membranes for used in guided tissue regeneration (GTR). The copolymer was synthesized by ring-opening polymerization (ROP) in bulk. The 3-dimensional fiber networks with built-in microporosity membranes were prepared by electrospinning. The pore size was varied between 5-30 μm and the porosity of membrane was 69%. After immersing in phosphate buffered saline (PBS), the membranes were degradable with time, as indicated by molecular weight loss, mass weight loss, reduction of pH of the media and changes in the surface topography and shape of the membranes. However, the in vitro hydrolytic degradation of the membranes was too fast for use as periodontal GTR. The membranes maintained their original shape for the first 4 weeks and the porous structure disappeared within 2 weeks. Results from agar diffusion test suggest the membranes to be non-cytotoxic. In conclusion, the electrospun membrane was non-cytotoxic but the degradation rate was too fast to be used as GTR membrane in periodontal treatment.