Papers by Author: Tao Qin

Abstract: Effect of NaH₂PO₄, Na₂HPO₄, MgSO₄, and Ascorbic acid on survival of Lactobacillus acidophilus was investigated before and after freeze-drying. Editing different concentrations of protective agents respectively during freeze-drying. After completion of the pilot, the survival ratio of Lactobacillus acidophilus and the number of viable cells were being measured. Results were as follows: the highest survival rate and the largest number of viable cells was Na₂HPO₄ as the protective agent.

Abstract: Four different prebiotics including fructo-oligosaccharide isomalto-oligosaccharide, inulin and xylo-oligosaccharide were screened for their effects on survival of Bifidobacterium bifidum during freeze-drying. The additions of prebiotics (v/v) were all 4%, 8%, 12%, 16% and 20%. The results showed that prebiotics could act on Bifidobacterium Bifidum. The optimum concentration of fructo-oligosaccharides (FOS) in samples for surival of Bifidobacterium bifidum was 12% and for viable count was 20％. Addition of xylo-oligosaccharides (XOS) played a significant role in promoting cell viablity when it was in low level. The optimum concentration of isomalto-oligosaccharides (IMO) for surival of Bifidobacterium bifidum was 16% and for viable count was 12％, while inulin in samples for surival and viable cell of Bifidobacterium bifidum both was 4％.

Abstract: In the present study, the experiments were investigated with effecting of sucrose, lactose, glucose, maltose on survival of Lactobacillus acidophilus during freeze-drying. In order to reach sufficient numbers viable cells, so different concentrations of protective agents were designed in the experiment, which as 3%, 6%, 9%, 12% and 15%, respectively. Measuring the survival ratio of Lactobacillus acidophilus and number of viable cells after completed drying. There were significant differences in survival during freeze-drying. Results showed that the protective agent was a critical factor affecting the survival of Lactobacillus acidophilus. In the experiments, the highest survival rate was soluble starch as the protective agent, and when the protective agent as galactose the number of viable cells was largest.

Abstract: Effect of the extract of Fructus tribuli (EFT) on growth of Lactobacillus acidophilus LA04, LA05, LA06 and LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS culture media as the control. The addition of EFT (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of EFT could promote the growth of Lactobacillus acidophilus LA09, The optimum concentration of EFT in MRS media was 1% at incubation 24h, but addition of EFT on growth of Lactobacillus acidophilus LA04, LA05 and LA06 has no significant effect.

Abstract: Effect of steroidal saponins fromFructus tribuli on growth of Bifidobacterium bifidum BB01, BB03 and Lactobacillus acidophilus LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The concentration of steroidal saponins (w/v) added in was 0.05%, 0.10%, 0.15%, 0.20% and 0.25%. Results were as follows: addition of steroidal saponins could improve the buffer ability of MRS and promote the growth of Bifidobacterium bifidum BB01 and BB03. The optimum concentration of nutrient supplement in MRS media was 0.20% at incubation 24h for Bifidobacterium bifidum BB01 and 0.25% at incubation 24h for Bifidobacterium bifidum BB03. Addition of steroidal saponins on Lactobacillus acidophilus LA09 didn’t play an obvious role in promoting growth when it was in high level.