Papers by Author: Yi Xuan Wang

Abstract: As a very important material, the use of hydrogen is increasingrapidly. Because the main advantage of hydrogen is that it is a clean and renewable energy source/carrier with high specific heat of combustion and no contribution to the Greenhouse effect, and can be used in many industrial applications. Many countries are developing to reduce operational cost of bio-producing hydrogen technology. So the key to realize industrialization is to improve biohydrogen-producing capacity and reduce the cost. This article discusses fermentative hydrogen production from various points of view. First, the theoretical principles of the biological processes taking place in hydrogen production, and the organisms responsible for this process are described at the same time. Second, Laboratory Experiments of fermentative hydrogen production are introduced. Types of fermentative and applicable reactor designs are discussed. Finally, the challenges faced by fermentative hydrogen production are discussed.

Abstract: Anaerobic process of biohydrogen production was developed recently. The representative strain of hydrogen production bacteria, namely fermentative hydrogen producing bacterial strain B49，was isolated from the activated sludge which was cultured artificially by our lab. In this study, there were some different C/N ratios employed. Experiment results indicated that the concentration of carbon and nitrogen had a great effect upon hydrogen production of B49, when C/N=2.5, glucose got the highest degraded efficiency, and at a C/N ratio of 3.3, the bio-gas and hydrogen production peaked with 159mL and 37.5mL, respectively, which considered that the optimum C/N ratio on the growth and hydrogen production was 3:1. Furthermore, the mainly terminal liquid products of B49 were ethanol and acetic acid. With the increase of the total organic nitrogen, final pH value decreased gradually.

Abstract: To develop the identification of species for fermentative biohydrogen-producing bacterium, scholars have found a method which is based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic regions. In the study, a large fragment of the rDNA operon, including the 16S rDNA, the intergenic spacer region (ISR) and approximately 2000 bases of the 23S rDNA, were polymerasechain reaction (PCR) amplified. The PCR amplification of the genomic DNA of Leptonema ilk strain 3055 using primers directed against conserved regions of the rRNA operon provided evidence that the 16S and 23S rRNA genes were linked via an intergenic spacer region. The sequencing of the intergenic spacer region indicated that it was 435 nucleotides in length and sequence similarity searches revealed that it bore no homology to any known sequences including tRNA available in databases.