Papers by Author: Ying Gao

Abstract: In this paper, a sensitive determination method for atropine based on end-column electrochemiluminescence of tris (2,2’-bipyridyl) ruthenium (II) detection is described. The conditions affecting separation and detection were investigated. Favorable ECL intensity of atropine was achieved in a solution consisting 5 mmol/L Ru (bpy)₃²⁺ and 50 mmol/L phosphate at applied voltage of 1.20V. The standard curve was linear between 1 and 20 μmol/L for atropine. The calibration equation and regression coefficients were: y = 128.38x−36.76 and R = 0.998 in terms of peak height response as a function of analyte concentration. A detection limit of 5×10⁻⁸ mol/L (S/N= 3) was achieved. The proposed method was applied satisfactorily to the determination of atropine in three pharmaceutical preparations.

Abstract: An electrochemiluminescence (ECL) sensor for highly sensitive detection of diphenhydramine was developed by employing tris (2,2-bipyridyl) ruthenium (II) as the detector. The ECL sensor was fabricated by immobilizing ECL reagent Ru (bpy)₃²⁺ on the poly-(3,4-ethylene dioxythiophene) and polystyrene sulfonate composite film (PEDOT-PSS) modified indium tin oxides (ITO) electrode by electrostatic attraction strategy. The ECL sensor showed an increased ECL intensity in the presence of diphenhydramine. The increased ECL intensity was direct proportion to the concentration of diphenhydramine over a range from 5.0 ×10^-4 to 5.0 ×10^-6 mol/L. The detection limit for the detection of diphenhydramine was 1.0 ×10^-6 mol/L. Fabrication of the ECL sensor by this method is simple and easy, and hence has potential applications in the field of ECL analysis and detection.

Abstract: Mn modified carbon sphere supported Pt catalyst was successfully prepared through a one-pot process. The modification of carbon sphere was operated in liquid phase reaction without any post-processing, and Pt was directly reduced to deposit on the composite support in the same suspension system. The role of Mn as a modifier can show change of adsorption state by XPS, XRD and electrochemical tests. At the same time, the improved the methanol electrooxidation performance, can be proved from lower adsorptive capacity and lower electrooxidation peak potential.

Abstract: The capillary electrophoresis (CE) is a powerful analytical technique in analytical chemistry and bioanalytical chemistry due to its high resolution, minimal sample requirements and low operating costs. Recent advances of application of CE for the analysis of traditional Chinese medicine is critically reviewed in this paper. After a simple discussion of the mode of separation and detection, special attention is focused on application of CE techniques and strategies for the qualitative and quantitative analysis in traditional Chinese medicine.

Abstract: Study of the electrochmeical biosensor has become a new interdisciplinary frontier between biological detection and material science due to their excellent prospects for interfacing biological recognition events with electronic signal transduction. Nanomaterials provided a significant platform for designing a new generation of bioelectronic devices exhibiting novel functions due to their high surface-to-volume ratio, good stability, small dimension effect, good compatibility and strong adsorption ability. In this paper, we review the development of electrochemical biosensors fabricated with various nanoscale materials, also highlight the analytical applications in terms of biochemistry.

Abstract: Nanomaterials include nanometal, nanooxide, carbon nanotube, carbon nanotube composite and other types of nanomaterials. They provide a significant way for the manufacture of sensors due to their small size, larger specific surface area, surface atomic slots insufficiency and more active sites. The rapid development of electrochemical sensor fabricated with nanomaterials is of significant value. In this article, we address some of the main advances in this field over the past few years, discussing the research progress of electrochemical sensor fabricated with nanomaterials and their application.

Abstract: A Photoelectrochemical Biofuel Cell (PEBFC) generating electrical energy directly from sunlight and biomass was investigated. In this paper,the PEBFC had a triarylamine dye-sensitized titanium dioxide(TiO₂) film photoanode and Pt black cathode. The electron transport process of the PEBFC was also described. The performances of the PEBFC were obtained by photocurrent–photovoltage characteristic curves.The open-circuit photovoltage(V_oc), the short-circuit photocurrent (I_sc) and the maximum power density (P_max) was 0.82V, 69.00 μAcm^-2 and14.00μWcm^-2 at 0.27 V, respectively. The fill factor (FF) was 0.25 and the incident photo-to-current efficiency (IPCE) was 3.5% at 441 nm.

Abstract: A fast method for the separation and determination of the food additive propyl gallate has been established by using capillary electrophoresis. The effects of several factors such as the applied potential and detection running buffer were investigated in order to obtain the optimum conditions, and the assay results were satisfactory. The limit of detection for the analyte was 10-6 mol/L. This approach has remarkable advantages with respect to other methodologies involving separations and electrochemical detection including minimal sample consumption, higher analysis speed and lower cost. In order to demonstrate the capabilitiy of the method, the determination of additive in a commercial food sample is also presented.

Abstract: A sensitive determination method for pethidine based on capillary electrophoresis-electrochemiluminescence detection was described. Analysis conditions affecting separation and detection were discussed and optimized in detail. The mixture solution of 5 mmol/L Ru(bpy)3²⁺ and 50 mmol/L phosphate buffer at pH 7.46 were added to the detection reservoir. End-column detection of pethidine was performed. Sensitive electrochemiluminescence detection was obtained at applied voltage of 1.20 V. A baseline separation of pethidine was achieved using a phosphate running buffer at pH 7.46 by electrokinetic injection for 10 s at 10 kV. A detection limit of 5.0×10^-8 M (S/N=3) was achieved.

Abstract: A capillary electrophoretic assay for determining synthetic antioxidant butylated hydroxyanisole in food has been developed. The extraction with 70% (v/v) methanol quantitatively extracted synthetic antioxidant. The separation was carried out by CZE using phosphate at a separation potential of 18 kV. Amperometric detection was achieved with an applied potential of 0.60 V. A linear relationship between the peak height and the concentration of the analyte was found in the range 1.8-180 µg/mL for BHA, with correlation coefficient of 0.994. The relative standard deviations of migration time and peak height were 0.19 and 5.3 %, respectively. The method developed was successfully applied for the determination of synthetic antioxidant butylated hydroxyanisole in food. Recovery of butylated hydroxyanisole was 93%.