Authors: Rui Ying Lei, Yue Wang, Yong Feng Li
Abstract: The beet sugar wastewater is a substance produced in the sugar process, which contains deep color and high concentration of nitrogen phosphorus and potassium. Treatments of beet sugar wastewater have been one of the most important challenges for environmental protection workers. Currently, treatments which are widely used in beet sugar wastewater processing are concentration drying, anaerobic method, aerobic method and incineration. The fundamental structure, working principle as well as hydraulic model of internal circulation (IC) reactor were introduced. The application and the possibility of using IC reactor to treat beet sugar wastewater were also concluded.
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Authors: Bi Da Qin, Xuan Dong Dong, Jia Yu Wang, Cai Yu Sun, Dong Pu Guo, Yong Feng Li
Abstract: The voltage is the critical electrochemical parameter in microbial fuel cells (MFCs).There are three major oilfield wastewaters including water flooding produced water, polymer flooding produced water and ASP flooding produced water. These three wastewaters were used as anode substrate of three MFCs in this study. The influence and the influencing factors of the output voltage of the three MFCs and the produced water main refractory organics removal effect were studied. The results show that During this reaction period, MFCs cathode potential stays relative stable, however, anode potential shows a remarkable increasing trend, thus, the anode contributes mostly to the change in output voltage. COD removal effect of Anode substrate and the coulombic efficiency are both influencing factors of the anode potential .The microbial fuel cell for wastewater of surfactant, remove the best effect;For different produced water, oil and polymer removal effect abide by the MFC output voltage and COD value changing law.
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Authors: Rui Ying Lei, Yue Wang, Qian Wei Liang, Yong Feng Li
Abstract: The principle and engineering control parameters of anaerobic bio-hydrogen in an up-flow anaerobic sludge blanket reactor are introduced, especially on the research situation and development trend of this technology. Many theoretical achievements of bio-hydrogen production using UASB reactor have been made, but the large scale industrialization of this technology has not been realized because there are still some difficulties in start-up and operation of the UASB reactor. Finally, some strategies and suggestions are proposed as guidance for further research.
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Authors: Jian Hui Zhao, Ning Li, Yong Feng Li
Abstract: The influence of organic loading rates (OLRs) on the production of fermentation hydrogen was investigated in a continuous stirred tank reactor (CSTR) with brown sugar water as the fermentation substrate, and sewage sludge as the initiation of reaction. Six OLRs were examined, ranging from 12 kg/m3·d to 32 kg/m3·d. The biogas and hydrogen production rates continuously increased with increasing OLR (12 kg/m3·d to 32 kg/m3·d).It reached a maximum production rate of 18.6L/d and a hydrogen production rate of 6.4L/d at OLR= 32 kg/m3·d. Compared with the initial 12kg/m3·d, gas production improved by 89% and 87%, respectively. During system operation, the reactor could maintain a high hydrogen production rate of ethanol-type fermentation by adding a certain amount of NaOH in the reactor to regulate the pH level.
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Authors: Dong Xing, Yong Feng Li, Li Wei, Jing Wei Zhang
Abstract: Most Oilfield of China has been a stage of Oil recovery with high water, microbial residual oil gasification technology as the oilfield's reserves technology has a good application prospect, especially the use of microorganisms for gasification of residual oil. The study has far-reaching significance, and it mainly turns the difficult mining oil reservoir into natural gas (mainly methane) through microbial degradation. It is the most effective, economical and environment-friendly way to enhance oil recovery efficiency and to extend the reservoir life. This paper summarized the relevant principles of oil microbial degradation and gasification, microbial enhanced oil recovery and Residual oil gasification at home and abroad, and come up with a few new research ideas.
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Authors: Yong Feng Li, Jian Yu Yang, Guo Cai Zhang
Abstract: A2O process is shortened form Anaerobic-Anoxic-Aerobic process, which can achieve carbon, nitrogen and phosphate removal, is widely used for its low cost and high efficiency. The Experiment using a 52.15 L anaerobic-anoxic- aerobic (A2O) reactor with simulate synthetic wastewater, by adjusting the aeration of Aerobic units, observing the performance of nitrogen and phosphorus removal under different dissolved oxygen(DO). The result of the whole operation show that the system can not keep a high TN removal efficiency under high aeration, and the low aeration made the system a significant decline in nitrogen and phosphorus removal.
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Authors: Jing Wei Zhang, Wei Han, Jian Yu Yang, Zhan Qing Wang, Yong Juan Zhang, Yong Feng Li
Abstract: The important premise for hydrogen production technology of fermentation generator was to separate and identify the efficient hydrogen producing bacteria. By use of Hungater technology and flat-panel technology with wide-body narrow-neck flask, LM-1 and HPB-LR medium to separate and identify the anaerobic fermentation hydrogen production bacteria, and five hydrogen-producing bacteria were obtained, whose hydrogen metabolism was ethanol fermentation. The product analysis on the hydrogen production bacteria fermentation liquid showed that ethanol and acetic acid accounted for 95%-99% of the total metabolites. These strains can be fermented to produce hydrogen, and can be used for industrial applications.
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Authors: Yong Feng Li, Yi Tong Duan, Fang Jing Liu, Lu Wang, Yi Xuan Wang
Abstract: Anaerobic process of biohydrogen production was developed recently. The representative strain of hydrogen production bacteria, namely fermentative hydrogen producing bacterial strain B49,was isolated from the activated sludge which was cultured artificially by our lab. In this study, there were some different C/N ratios employed. Experiment results indicated that the concentration of carbon and nitrogen had a great effect upon hydrogen production of B49, when C/N=2.5, glucose got the highest degraded efficiency, and at a C/N ratio of 3.3, the bio-gas and hydrogen production peaked with 159mL and 37.5mL, respectively, which considered that the optimum C/N ratio on the growth and hydrogen production was 3:1. Furthermore, the mainly terminal liquid products of B49 were ethanol and acetic acid. With the increase of the total organic nitrogen, final pH value decreased gradually.
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Authors: Yi Tong Duan, Fang Jing Liu, Lu Wang, Yong Feng Li
Abstract: Biohydrogenbacterium R3 sp.nov, as a typical strains of hydrogen production bacteria, was isolated from the activated sludge in the biohydrogen production reaction by using culture media of HPB-LR which was designed particularly for isolating anaerobic fermentation bacteria and an improved Hungate rolling tubes technique and a plate method of culture bottle (PMCB) which were used for counting and isolating the anaerobes by our lab. Two representative strains of hydrogen production bacteria, namely RL20, RL37, and a strain of no hydrogen production bacterium, namely RL16 were used as contrast analysis. Experiment results indicated that the distributed proportion of ethanol and acetic acid was 95%-99% in the total amount of VAFs (Volatile Fatty Acid) of R3 which were typical ethanol-type fermentation, and the total amount of ethanol and acetic acid was commensurate with that of propionic acid.
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Authors: Li Ran Yue, Yong Feng Li, Nan Qi Ren, Yong Ming Hui, Jian Zheng Li
Abstract: Biohydrogen production technology by fermentation uses biomass as raw materials to produce the renewable hydrogen energy. H2-producing bacteria(HPB) plays an important part in Biohydrogen production technology by fermentation。In this paper study on growth and Hydrogen production characteristics of HPB B49.Research has shown:⑴.Glucose concentration 10 g/L was the fittest substrate concentration of HPB B49 growth and hydrogen production;⑵Enzyme system growth of hydrogen-producing bacterium B49 at different growth phases influences hydrogen production;.⑶35 was the fittest temperature for cell growth and hydrogen production;⑷The fittest growth pH for hydrogen producing bacterium B49 was 4.5, while that for cell hydrogen production was 4.0;(5). This bacterium strain could be used as engineering bacterium in the different substrate hydrogen-producing experiments.
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