Papers by Author: Yu Bin Ji

Abstract: To study the mitotic catastrophe induction effect of chelidonine on human gastric carcinoma SGC-7901 cells, MTT assay was applied to detect the proliferation inhibition effect of chelidonine. Giemsa staining was used to observe the morphological changes of the nucleus in gastric cancer cells and mitotic index (MI) was calculated. The results showed that chelidonine could effectively inhibit the proliferation of SGC-7901 cells and the IC₅₀ was 48.67μmol/L. After treatment with 6.25-25μmol/L chelidonine, the numbers of giant cells with two or more nuclei was increased along with the increase of drug concentration. The mitotic index of cells was significantly increased compared with that of the control group (P<0.01). The conclusion can be drawn that chelidonine can inhibit the proliferation of SGC-7901 cells by inducing mitotic catastrophe of gastric carcinoma cells.

Abstract: To study the effect of chelidonine on Cdk1, p-Cdk1( Thr14), cyclinB1 protein expression in SGC-7901 Cells, and to explore the mechanism of chelidonine inducing G₂/M phase arrest in SGC-7901 Cells. Western Blotting was used to study the effects of chelidonine on the Cdk1, p-Cdk1(Thr14) and cyclinB1 protein expressions in SGC-7901 cells. Chelidonine could significantly decrease the expression of Cdk1 and cyclinB1 protein. Meanwhile, chelidonine could remarkably increase the expression of p-Cdk1(Thr14) protein in a dose dependent manner. Chelidonine can down-regulate the expression of Cdk1, cyclinB1 and up-regulate p-Cdk1 (Thr14) protein expression in SGC-7901 cells,which may be one of the role mechanism of G₂ /M phase arrest induced by chelidonine in SGC-7901 cells which may be one of the role mechanism of G₂ /M phase arrest induced by chelidonine in SGC-7901 Cells.

Abstract: Lycorine is a kind of natural alkaloid extracted from Amaryllidaceae that has shown various pharmacological effects. Recent studies have focused on the potential antitumor activity of lycorine. The inhibition effects of lycorine on the cell proliferation of leukemia HL-60 cells, K562 cells, chronic lymphocytic leukemia, U937 cells and structure-activity studies on the lycorine pharmacophore in leukemia cells were summarized in the article. The review of pharmacological effects and structure-activity on leukemia of lycorine can provide a reference for the further development and utilization on the kind of natural products.

Abstract: Narciclasine (NCS) is a plant growth inhibitor isolated from the secreted mucilage of Narcissus tazetta bulbs, which is commonly used anticancer agent in human systems. Refer to the literatures in recent years, this article reviewed the anti-cancer effects of potential anticancer drugsNarciclasine, which induces apoptosis by activation of the death receptor and/or mitochondrial pathways in cancer cells. Narciclasine displays antitumor effects in apoptosis-resistant as well as in apoptosis-sensitive cancer cells by impairing the organization of the actin cytoskeleton.

Abstract: The purpose of this study is to investigate the extraction and separation of alkaloids from H.littoralis. Chopped bulbs of H.littoralis was soaked in a mixture of dichloromethane and methanol (1:1) for 24 h, ultrasonic extraction six times, then extracted with N-butanol to yield crude extracts. Three components were isolated from N-butanol extract of H.littoralis by Sephadex LH-20 and TLC. The compounds were identified by LC-MS, they are 5,6-dihydrobicolorine, Lycorine and 7-deoxy-trans-dihydronarciclasine.

Abstract: socarbostyril alkaloids is a kind of alkaloid does not contain basic nitrogen atoms and is represented by hydroxylated benzophenanthridone or isoquinolinone types of structure. The most widely known compounds of this group are narciclasine, lycoricidine , and pancratistatin. They have demonstrated to inhibite the proliferation of many human cancer cells, and at the same time have no affect on normal human cells under a certain dose, they have a high efficiency and low toxicity in antitumor area. Now this kind of compound has been a hot spot research to antitumor workers. The present paper reviews the origin and the antitumor function of the Isocarbostyril alkaloids.

Abstract: The purpose of this study is to investigate the effects of different extraction solvents and extraction methods on Dauricine concentrations in Asiatic Moonseed. Dauricine was extracted from Asiatic Moonseed by ultrasonic method with sulfuric acid and ethanol as solvents; then the comparative study was undertaken in the three extraction methods (warm immersion, percolation, ultrasonic) for Dauricine. The results showed that the extracting effect was best in the ultrasonic method with sulfuric acid as a solvent.

Abstract: This paper reviews the structure-activity relationship of two typical Amaryllidaceae isocarbostyril alkaloids, narciclasine, pancratistatin and their derivatives. Varieties structures have important impacts on biological activity of Amaryllidaceae isocarbostyril alkaloids, for example, the nuclear structure of isocarbostyril, the stereochemistry of the B/C ring junction, the C7-hydroxy of ring A, hydroxies of ring C and so on. This study is conducive to find other active groups, conduct structural modifications and provide the rational basis for designing drugs molecular.

Abstract: This paper investigated the selenylation, characterization and cytotoxic activity of seleno-Astragalus polysaccharide. Firstly, Se-APS was synthesized under reaction time at 8 h, reaction temperature at 80 °C, ratio of Na₂SeO₃ to APS at 1.0 g/g and water bath shaking rate at 40 r/min. Then, a series of experiments were designed to investigate the characterization and cytotoxicity of Se-APS. The result indicated that the characterization of Se-APS was significantly different from APS, except for X-ray diffraction. Additionally, MTT assay conformed that Se-APS could significantly inhibit the proliferation of human breast cancer MCF-7 cells in dose-dependent and time-dependent manners.

Abstract: To explore the influence of Asparagus polysaccharide on Band 3 proteins and glycophorin A(GPA) of S180 mice erythrocyte membrane by contrasting the groups treated with deferent ways,The experiment was equipped with 5 groups: normal control group (physiological saline), negative control group (physiological saline), Asparagus polysaccharide group (25mg/kg), Asparagus polysaccharide group (50mg/kg) and Asparagus polysaccharide group(100mg/kg).The content of sialic acid(SA) was determined by spectrophotometric method; the negative charges were detected by high performance capillary electrophoresis(HPCE); the relative content of glycophorin A and Band 3 were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE; anion transportation function of Band 3 was examined by the fluorescence spectrophotometric method;Flow Cytometer(FCM) was needed to observe the potential of erythrocyte membrane. In the result, Asparagus polysaccharide increased the content of sialic acid; advanced the migration time; improved the relative content of GPA and Band 3; obviously enhanced the CI- transportation function of erythrocyte Band 3; and caused the membrane potential obviously to elevate, Asparagus polysaccharide has the simultaneously influence on the functions of glycophorin A and Band 3 which are closely connected. It is assumed that the anti-tumor function of Asparagus polysaccharide partly concerns function adjustments of glycophorin A and Band 3 from S180 mice erythrocyte membrane.