Papers by Author: Zhong Xu

Abstract: Some carbon nanomaterials have been proved to be able to improve the PCR amplification efficiency. If used in quantitative real-time PCR (qPCR), these nanomaterials must be tested whether fluorescence processing is interfered after they are added in the PCR system. In this study, 76 different carbon nanomaterials were tested in SYBR Green I-based qPCR, and the results demonstrated that about half carbon nanomaterials tested in this study could alter the PCR amplification profile probably due to the fluorescence quenching. Surprisingly, lower concentrations of nanomaterials led to more slight interference with the melting temperature.

Abstract: A Gram-positive, strictly aerobic and moderately halophilic bacterial strain HSD was obtained from the sea water sample collected from International Sea Water Bathing Place in Weihai, a city on the shore of the Yellow Sea. Strain HSD was bacillus. The optimum growth temperature was 32~37°C and the optimum pH was 6.0~9.0. It could grow well at 100 g•L-1 salt mass concentration. The G+C mole fraction of its genomic DNA was 37.5%. 16S rRNA gene sequence indicated a close relationship to Alkalibacillus halophilus (DQ359731) with similarity of 99%. However, they had obvious difference in whole-cell main fatty acid components, cell size, cell morphology, motility, oxidase, gelatine liquefication, nitrate reduction, NaCl tolerance range, pH tolerance range, G+C mole fraction, sole carbon source, sole nitrogen source, antibiotic sensitivity and a source of the strain isolation. Comparing of these taxonomic characters of strain HSD with those of other type strains of the genus Alkalibacillus, the differences were more obvious. In view of multiple identification results, it was proposed that strain HSD was classified as the type strain of a new species of the genus Alkalibacillus under the name Alkalibacillus huanghaiensis sp.nov..

Abstract: A Gram-positive, strictly aerobic, spore-bearing and moderately halophilic bacterial strain HNPHT was obtained from the sea mud sample collected from Jinhaitan Park in Weihai, a city on the shore of the Yellow Sea. Strain HNPHT was bacillus. The optimum growth temperature was 30-36°C and the optimum pH was 6.5-9.5. It could grow well at 5-20% salt concentration. The G+C content of its genomic DNA was 37.0%. 16S rRNA gene sequence indicated a close relationship to Alkalibacillus halophilus (DQ359731) and Alkalibacillus salilacus (AY671976) with similarity of 99%. However, they had obvious difference in whole-cell main fatty acid components, cell size, cell morphology, colony color, motility, oxidase, gelatine liquification, nitrate reduction, NaCl tolerance range, pH tolerance range, G+C mole fraction, sole carbon source, sole nitrogen source, antibiotic sensitivity and a source of the strain isolation. A comparison of these taxonomic characters of strain HNPHT with those of some type strains of the genus Alkalibacillus indicated that the strain could be distinguished from A. halophilus and A. salilacus. In view of multiple identification results, it was proposed that strain HNPHT was classified as the type strain of a new species of the genus Alkalibacillus under the name Alkalibacillus weihaiensis sp. nov..