Papers by Keyword: Antioxidant

Abstract: Garlic is a globally used spice due to its pungent taste and a fishy deodorant and digestive stimulator. Owing to the widespread use of garlic in both Oriental and Western medicine, further investigations on garlic extracts might carry important implications in the industry. In this study, we conducted extraction of garlic extraction by two conventional methods and an ultrasonic assisted method. The processes were optimized with respect to various quality parameters including polyphenol, flavonoid content, and antioxidant activity. The results showed that extracting garlic under the ultrasonic support will show higher efficiency than the non-ultrasonic extraction. The optimal extraction parameters are: 70% ethanol solvent, 10/1 solvent / material ratio, 40 °C temperature and 40 minutes time. Keywords: Allium Sativum L., Garic, Polyphenol, Flavonoid, Antioxidant

Abstract: Abstract The aim of this study was to fabricate curcumin-loaded polymeric mixed micelle which was a new nanocarrier of therpeutic agent for skin uses. Curcumin was extracted from dried turmeric rhizomes using ethanol and recrystallized. The purity of curcumin was 79±3.6 %w/w. Six curcumin-loaded polymeric micelles (PM1-PM6) were prepared by simple dissolution method using poloxamer 407 (5% and 10%) as a main core structure. PEG-40 hydrogenated castor oil (PEG-40HCO) was incorporated at two percentages (2.5% and 5.0%) to study the effect on the nanoparticle characteristics. The average particle sizes of PM1-PM6 were in the range of 33.3±6.6 nm to 171.3±52.8 nm. The entrapment efficiency and the loading capacity of curcumin were in the range of 47.45%-77.35% and 0.048%w/w-0.078%w/w, respectively. When PEG-40HCO was incorporated in to the polymeric micelles, the particle size decreased and the entrapment efficiency increased. Thus, PM4 and PM5 were selected for further study. Moisturizing antioxidant creams containing 0.005%w/w of curcumin loaded in PM4, PM5 and curcumin simply dissolved in propylene glycol (PG) were formulated. The resulted formulations showed good spreadability and good characteristics. After being subjected to accelerated test, all of the formulations remained with characteristic color, pH and showed no phase separation. The stability data showed that the moisturizing antioxidant creams were stable for the whole 3 months after storage at accelerated temperature (45°C/75%RH). The study demonstrated that polymeric mixed micelle spontaneously encapsulated a poorly water-soluble curcumin and increased the solubility up to 250 folds. The developed moisturizing cream containing 0.005%w/w of curcumin resulted a greenish-yellow color preparation. It had tolerable physicochemical properties based on curcumin content, pH and viscosity under the harsh condition. The cream also had satisfactory antioxidant activity, which can be regarded as an effective and acceptable therapeutic or skincare products for topical uses.

Abstract: Mangiferin, a polyphenol of C-glycosylxanthone, exhibits various bioactivities with poor aqueous solubility. It is known as a potent antioxidant, which leads to remarkable UV protection and anti-aging properties. Mangiferin can be found in many plant species, among which the mango leaf is one of the primary sources. From our study, the extraction yield of mangifein obtained from the leaves of Mangifera indica L. variety Nam Doc Mai was 3.17% with 95.02% ± 0.064 purity (HPTLC analysis). The solubility of mangiferin in the studied pure solvents arranging in descending order were ethoxydiglycol, dimethyl isosorbide, polyethylene glycol 400, polyethylene glycol 600, propylene glycol, dipropylene glycol, glycerin, isopentyldiol, methanol, ethanol and water, whereas the addition of the solvent in water could increase the aqueous solubility of mangiferin. In several cases, the solubility was apparently higher than that dissolved in its pure solvent state. The log-linear solubility model for the cosolvent system was used to calculate the volume fractions of the selected solvents needed to solubilize mangiferin content at the twenty times of the IC₅₀ against DPPH radicals. In conclusion, the developed aqueous formulation contained 0.5% w/v of mangiferin and 20% w/v of polyethylene glycol 600 or dipropylene glycol as a solubilizer in water.

Abstract: The black bamboo leaves that have been widely used by the community for a long time are thought to have bioactive compounds. To find out more, it is necessary to evaluate the levels of phenolic compounds and flavonoids which are reported to function as antioxidants and antimicrobials. In addition, antioxidant and antimicrobial activity tests also need to be carried out directly on black bamboo extracts from Indonesia. This study aims to evaluate the phenolic and flavonoid levels of a black bamboo leaf extract from Indonesia. Based on the research results, the total phenol content of the ethanol extract of bamboo leaves (Bambosa sp.) was 157.426 mg gallic acid extract (GAE)/g and the total flavonoid content was 19.406 mg equivalent to quercetin extract (QE)/g. The antioxidant activity using the DPPH method obtained IC50 of ethanol extract of bamboo leaves (Bambosa sp.) of 46.8672 μg/mL, and an IC50 BHT value of 9.5454 μg/mL. Based on these results, bamboo leaves (Bambosa sp.) have the potential to be developed as antioxidants and antimicrobials.

Abstract: Chalcone has a variety of interesting biological activities, including as an antioxidant and anticancer. Antioxidants are molecules that can retard or prevent the oxidation process or inhibit the formation of free radicals. p-Hydroxy-m-Methoxy Chalcone (pHmMC) is a chalcone derivative reported has anticancer activity both in used single and in combination treatment with Doxorubicin (DOX) in breast cancer cell lines. DOX is one of the chemotherapy agents widely used in cancer treatment but the medicine has side effects of cardiotoxicity. This effect is generally associated with free radical formation. This study aims to find out the activity of pHmMC as an antioxidant both in used single and in combination treatment with DOX by in vitro and to explore the potential of pHmMC as an antioxidant by in silico. Activity tests as antioxidants were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. Ascorbic acid (Vitamin C) was used as a positive control. Antioxidant activity was calculated as the value of 50% Inhibition Concentration (IC₅₀). In silico study was carried out by molecular docking using Protein Ligand ANT System (PLANTS) software with peroxiredoxin 5 [1HD2] as the target. The results showed that the IC₅₀ values of pHmMC, DOX, and Vitamin C were 11.9; 21.6 and 3.3 μ/mL respectively. The combination of pHmMC-DOX has a higher antioxidant activity than single pHmMC or single DOX. The docking molecular showed that there were similarities amino acids involved in the interaction between PRDX5[1HD2]-pHmMC and PRDX5[1HD2]-DOX. This research indicated that pHmMC and pHmMC-DOX are potentially developed as an antioxidant.

Abstract: Moringa oleifera (M. oleifera) has been known as a medicinal plant. The objectives of this study is to identify the chemical compositions of M. oleifera pods extracts and to evaluate its total phenolic content (TPC) and antioxidant activity. The methodology of this study began with the collection of the pod's sample, drying and grinding the pods into powdered form. M. oleifera pods extracts were prepared by using methanol (MeOH), hexane, dichloromethane (DCM), and ethyl acetate (EtOAc) solvent which yielded respective residues. All extracts were subjected to Gas Chromatography-Mass Spectrometry (GC-MS) to identify its chemical compositions. Major compound found in hexane, DCM, EtOAc, and MeOH extracts were nonanoic acid (30.94 %), diisooctyl phthalate (89.45 %), benzoic acid, 4-hydroxy- (37.57 %) and octanoic acid (19.19 %), respectively. The TPC quantification was done via Folin-Ciocalteu method showed that EtOAc extract has the highest phenols content with 500.05 mg GAE/g. Generally, all extract contain moderate phenolic content. M. oleifera pods extracts were screened for the antioxidant activity via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. Hexane, DCM and EtOAc extracts exhibited good free radical scavenging activity with IC₅₀ of 65.79 μg/mL, 74.93 μg/mL and 82.83 μg/mL, respectively. MeOH showed the lowest free radical scavenging activity with IC₅₀ 529.6 μg/mL. However, antioxidant activity of all extracts was low compared to standard, ascorbic acid (AA) with IC₅₀ of 19.57 μg/mL. The presence of various bioactive constituents in the M. oleifera pods extracts might contributed to the antioxidant activity of the plant.

Abstract: The antioxidant capacity by DPPH radical scavenging and antimicrobial activity by disk diffusion and broth microdilution method of nine isolated alkaloids from T. crispa were evaluated. All isolated alkaloids had been divided into three groups which were aporphine alkaloids, N-formylannonaine (1), N-formylnornuciferine (2), magnoflorine (9), oxoaporphine alkaloids, lysicamine (3) and liriodenine (4); and protoberberine alkaloids, columbamine(6), dihydrodiscretamine (7) and 4,13-dihydroxy-2,8,9-trimethoxydibenzo [a,g]quinolizinium (8). Protoberberine alkaloids showed (IC₅₀ > 500-800 μg/mL) radical scavenging activity while oxaporphine alkaloids inhibited the growth of the Gram-positive bacteria, Staphylococcus aureus (+) and Enterococcus faecalis (+).The antioxidant and antimicrobial properties of different compounds support documented traditional use of T. crispa in wound healing and treatment of rheumatic, diarrhoea, ulcers, itches and wounds. Results of the present biological activity investigation further points to the potential of this plant species as a good source of natural antioxidant and preservative in food industry.

Abstract: The use of natural rubber-like crepe rubber as an asphalt additive is very susceptible to aging. Aging on asphalt crepe rubber can occur during the mixing process or its use for the road. Studies on the prevention of aging on asphalt are still being developed to produce asphalt resistance to aging and have good performance in preventing deformation. Some studies that have been done are the addition of other additives such as antioxidants. This study aims to study the effect of adding crepe rubber and the antioxidant 1,2-dihydro-2,2,4-trimethyl-quinoline (TMQ) to the Marshall characteristics of rubber asphalt. Preparation of the rubber asphalt sample begins with making crepe rubber, which is by milling raw natural rubber in the form of a cup lump using a creeper. The crepe rubber product contains dry rubber content of more than 95%. Then the crepe rubber masticated using an open mill to soften and form it with 2 mm thickness. Then the crepe rubber is melted at 200 °C before being mixed with asphalt, heated to 165 °C. The crepe rubber content in the asphalt mixture is made 10%, and during the mixing process, the TMQ was added with contents of 0%, 1%, 2%, 3% w/w. The rubber asphalt samples were then tested for their penetration and marshall characteristics, using ASTM D5 and ASTM D6927 standards. The test results show that the addition of the TMQ can improve the penetration properties and stability of the rubber asphalt in holding the load. However, the addition of the TMQ of more than 2% causes a decrease in the rubber asphalt's stability properties. The best results were obtained by adding 2% TMQ with 68.7 dmm penetration and optimum asphalt content of 5.50%. Results of the marshall test for the sample are marshall stability 1403.96 kg, void filled aggregate (VFA) 75.90%, void in a mixture (VIM) 3.07%, void mineral aggregate (VMA) 15.34%, flow 3.370 mm, and marshall quotient (MQ) 420.8 kg/mm.

Abstract: Fresh whole flowers of the butterfly pea were extracted with 95% ethanol and analyzed the antioxidant activity. The total phenolic content (TPC) of butterfly pea ethanolic extract (BE) was 26.90±1.12 mg gallic acid equivalents/g of dry weight and radical DPPH scavenging activity of ethanolic extract was. The ethanolic extract-loaded Nanostructured Lipid Carriers called BE-NLC were prepared by the high shear homogenization method using compritol 888 ATO, miglyol oil, poloxamer 188, extract and tween 80, respectively. Particle size, polydispersity index, zeta potential 27.78±0.12 mg Trolox equivalents/g of dry weight and entrapment efficiency (EE) were determined. The morphology of the BE-NLC was spherical. The entrapment efficiency of extract was 72.51±1.11% in NLC system. Antioxidant activity was evaluated by the estimation of peroxide values (PVs) of fish oil from catfish, treated with natural antioxidant (BE and BE-NLC) and compared with butylated hydroxy toluene (BHT) as synthetic antioxidant by incubating at 65 °C for a period of 12 days. BHT was found to have higher antioxidant activity than BE and BE-NLC in fish oil. Treatment containing BE-NLC and BHT in fish oil showed similar PVs after the 4 days. However, the antioxidant activity of BE-NLC in stabilization of fish oil is higher than that of BE.

Abstract: Maclura cochinchinensis Corner or Kae Lae in Thai belongs to the Moraceae family. The plant is found in Southeast Asian countries and used in traditional medicine as a tonic agent to treat chronic fever and skin infections. Previous studies have demonstrated that plants in Moraceae family, such as Morus alba and Artocarpus lakoocha are rich source of antioxidant and anti-tyrosinase agents. Thus, the current study aimed at comparing different solvent extracts (50% ethanol, 80% ethanol, methanol by ultrasound and water decoction) of M. cochinchinensis heartwood for their antioxidant and anti-tyrosinase activities. Total phenolic content, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and anti-lipid peroxidation assays were performed to compare the antioxidant activity, whereas, Dopachrome method was performed for anti-tyrosinase activity. The results demonstrated that 80% ethanol and methanol extracts had better antioxidant activities based on EC₅₀ value of DPPH (9.96±0.21 and 9.50±0.46 μg/mL, respectively), ABTS (6.54±0.13 and 6.58±0.69 μg/mL, respectively), anti-lipid peroxidation (228.31±2.03 and 231.43±1.41 μg/mL, respectively), and anti-tyrosinase (7.60±0.29 and 7.91±0.41 μg/mL, respectively) assays. Further, 80% ethanol extract was selected to evaluate the bioactive compound using bioactivity-guided isolation. Oxyresveratrol identified as bioactive compound, exhibited higher activities than the positive controls (quercetin and kojic acid). In conclusion, 80% ethanolic M. cochinchinensis heartwood extract obtained using the ultrasound technique exhibited strong antioxidant and anti-tyrosinase effects and can be used as a potential active ingredient for cosmeceutical products. Oxyresveratrol could be used as bioactive marker for quality control of the extract and products.