Papers by Keyword: Antiviral Activity

Abstract: In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pUC19-His expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-α was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 1 mM IPTG at 37 °C, the expressed protein was inclusion body. His-chIFN-α was purified by Ni-metal chelate affinity chromatography. His-chIFN-α was shown to inhibit the replication of Newcastle disease virus in CEF cells.

Abstract: The effect of the processing conditions of dolomite powders on the antiviral activity was studied against H5N3 avian influenza virus. When dolomite was fired in the ambient atmosphere, it decomposed into MgO and CaO by two stages at different temperatures, namely into MgO+CaCO3 at ca.1010 K and MgO+CaO at ca. 1070 K. Strong antiviral activity was observed after the complete decomposition into CaO and MgO. However, when the firing temperature exceeded 1673 K, the antiviral activity was degraded. It was also found that the degraded antiviral activity was recovered by sever ball mill grinding.