Papers by Keyword: BSA Adsorption

Abstract: Surface modification of microporous polypropylene membrane was performed by grafting polymerization of acrylamide.The morphological and microstructure changes of the membrane surface were confirmed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and atomic force microscopy. The results indicate that the pore size of the grafted membrane was reduced.

Abstract: Protein adsorption is driven by various interactions. The contribution of surface charge to bovine serum albumin (BSA) adsorption on hydroxyapatite (HA) ceramic was investigated by adjusting the liquid environment in which the solid particles dispersed. Zeta potentials of HA and the adsorption of BSA on the surface were tested as a function of pH, ionic strength, Ca2+ and PO4 3- concentrations in the aqueous solutions, and the results showed that both of them were greatly affected by those experimental variations. Besides, the amount of adsorbed BSA was related to the variation of zeta potential of HA, as could be well understood in terms of electrostatic interactions.

Abstract: Studying on the interaction between proteins and calcium phosphate implants is one of the basic subjects in biomaterials science and engineering. In this work, zeta potentials and contact angles of hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramics were measured, and the adsorption behaviors of bovine serum albumin (BSA) on the surfaces were investigated. The adsorption isotherms of BSA on both ceramics follow the Langmuir type, however, BCP shows higher BSA adsorption ability. The differences of contact angles and zeta potentials in HA and BCP are the key factors to determine their ability to bind BSA molecules.