Papers by Keyword: Bio-Mimetic Synthesis

Abstract: Sodium hyaluronate / konjac glucomannan (SH/KGM) porous scaffolds were prepared via blending sodium hyaluronate and konjac glucomannan. The ammonia was used as cross-linker in blending process. The SH/KGM scaffolds were soaked into calcium nitrate solution and then followed by immersing into simulated body fluid to get SH/KGM/HAP porous scaffolds. X-ray diffraction and fourier transform infrared spectroscopy (FTIR) were used to characterize the crystallization and chemical structure of SH/KGM and SH/KGM/HAP scaffold materials. The scanning electron microscope (SEM) was used to analysis the morphology of SH/KGM/HAP scaffold and BMSCs on surface of the scaffold. The results show that hydroxyl-apatite produced on the surface of the SH/KGM, which appears as spherical particles in the SH/KGM/HAP scaffold surface, and the SH/KGM/HAP porous scaffold possesses good biocompatibility with cell.

Abstract: The bone tissue engineering scaffold was developed by compounded the type I collagen with the porous scaffold of the sol-gel derived bioactive glass (BG) in the system CaO-P2O5-SiO2. The resultant porous scaffold was treated in supersaturated calcification solution (SCS) to form the surface layer of hydroxyl-carbonate-apatite (HCA) since the type I collagen possessed good biocompatibility and bio-absorbability, and also, the ability of inducting calcium phosphates to precipitated inside and outside the collagen fibers where the collagen fibers acted as bio-macromolecules template for formation of bone-like inorganic minerals in nature bone such as: octo-calcium phosphate (OCP), tri-calcium phosphate (TCP) and hydroxyl-carbonate-apatite (HCA). On the other hand, the sol-gel derived bioactive glass also played an important role in formation of the above bio-minerals owing to its serial chemical reactions with the body fluid. The in vitro study in supersaturated calcification solution SCS indicated that the surface of the porous scaffold was able to induce formation of bone-like HCA crystals on the pore walls of the scaffold which possessed satisfactory cells biocompatibility.

Abstract: The spherical nano-sized bioactive particles in the system of CaO-P2O5-SiO2 were bio-mimetically synthesized using micro-emulsion method. The microstructures and properties of the bio- mimetic nano-materials were characterized using XRD, FTIR, SEM/EDAX and TEM techniques. It was indicated that the nano-particles possessed glassy structural characteristics. The porous composite for bone tissue reconstruction was prepared by compounding poly (hydroxybutyrate-2-co-2-hydroxyvalerate) (PHBV) and the nano-particles of bio-mimetic bioactive glasses (BMBG). Bone-like hydroxyl- carbonate-apatite (HCA) could formed on the surface of porous composite by immersing the composite in simulated body fluid (SBF) at 37°C for 8 hours. With increase of immersion time, the morphology of HCA changed from spherical into flake-like crystals. The study on cells attachment of the porous PHBV/BMBG composite proved that the material possessed satisfactory bioactivity, bio-mineralization function and cells biocompatibility.