Papers by Keyword: Blood Compatibility

Abstract: Titanium nitride (TiN) film on titanium film (Ti) was formed by magnetron sputtering method. Pure titanium substrates with TiN/Ti multi-layered films deposited using DC sputter-deposition machine in Ar gas atmosphere, in order to improve not only the blood compatibility of pure titanium but also the adhesion between the deposited TiN coating and the pure titanium substrate. The effects of the thickness of a pure titanium interlayer on adhesion of the TiN coating to the pure titanium substrate were investigated. And the effects of the TiN coating obtained in this study on blood compatibility were also investigated. The obtained multi-layered films looked yellow gold and appeared to be uniform and adhesive without any peel-offs. Based on the results of the platelet test, the ratio of the number of adhered platelets for the TiN/Ti film to that for the pure titanium substrate was estimated to be 0.54. Thus it was found that the platelet adhesion of the obtained TiN/Ti film was much smaller than the pure titanium, concluding that the TiN coating improved the blood compatibility.

Abstract: The anodic oxidation/TiO₂ composite film was prepared on the surface of AZ31B Mg alloy by DC magnetron sputtering. The corrosion resistance and blood compatibility of the film were systematically studied by electrochemical, dynamic clotting time and platelet adhesion test. The results shows that the corrosion current of AZ31B Mg alloy was 6.409×10-8A/cm2 after anodic oxidation treatment, which has decreased 4 orders of magnitude compared to the untreated samples and the corrosion resistance is improved greatly. The clotting time of anodic oxidation/TiO₂ film is about 53 min, which has increased 1.3 times compared to anodic oxidation film (40min). Platelets adhesion to anodic oxidation/TiO₂ film are less than the one adhesion to anodic oxidation film, and there are no pseudopodia and aggregation, which indicate that the blood compatibility of anodic oxidation/TiO₂ film is better than anodic oxidation film.

Abstract: In this paper, for the purpose of improving the hemocompatibility and surface tension of thermoplastic polyurethane (TPU) elastomers, poly butylene glycol ether hexafluoride fluoride ether glycol (PF) mixture with polytetramethylene ether glycol (PTMEG) was used as soft segments to synthesize a series of fluorinated thermoplastic polyurethane elastomers (FTPU) by two-step polymerization. The hemocompatibility in vitro was evaluated with platelet-rich plasma (PRP) contact tests. It was found that almost no platelet adhesion and activation was observed on the surface of FTPU elastomer when fluorine content was above 6wt% because of its low surface energy. The results indicated that fluorinated chain can be easily enriched on the surface of films because of higher C-F bond energy which due to greater micro-phase separation.

Abstract: The biocompatibility of the austenitic stainless steel with different nitrogen contents was studied, The MTT(3-(4,5-Dimethylthi azol-2-yl)-2,5-diphenyltetra zolium bromide) assay, platelet adhesion and hemolysis rate test were used to examine the cytotoxicity. The cell compatibility of materials was investigated by cell adhesion test. Hemolysis rate test and platelet adhesion were used to examine the blood compatibility. The cell toxicity test showed that the nickel free austenitic stainless steel materials of different nitrogen content and the control group of titanium alloy materials had no significant side effects for vascular endothelial cells. The cell adhesion test showed that the cell number of vascular endothelial in the austenitic stainless steel was more than the titanium alloy materials, and the cells grow in good condition. The hemolysis rate was lower than 5%, and the influence of platelet was not significant, and pyrogen test results can be considered that such material extracts heat checking compliance with safety regulations.

Abstract: Blood oxygenators play key role in Extra Corporeal Membrane Oxygenator (ECMO) system using for patients with acute respiratory problems, immature fetal and also in open heart surgery. Interaction between blood and blood oxygenator polymeric membrane surface lead to fouling phenomena which have negative effect on performance of this important medical device. A modification comprising surface activation, PEG immersing and PEG graft polymerization carried out to provide acceptable blood oxygenator performance, blood compatibility and reduction in heparin consumption at the same time. Modified membranes characterized by FTIR, contact angle measurements and Atomic Force Microscopy (AFM) analyses. Blood compatibility of modified surface was also detected by SEM images. Results clearly indicate that modifying membranes by PEG is an effective way for anti-fouling properties. Water contact angel reduction from 110ْ to 72ْ shows hydrophilicity enhancement, roughness increasing from 15 to 20 and blood compatibility improvement was investigated by SEM and AFM analysis results respectively.

Abstract: Purpose: The objective of this study was to evaluate the teraogenic toxicity of central vein catheters after irradiation sterilization and provide basis for further application of medical apparatus in future. Methods: central vein catheters were sterilized by irradiation as ISO11137 standard, afterwards, a series of tests including chromosome aberration test, micronucleus test and Ames test were performed to evaluate their teratogenic toxicity. Results: After irradiation sterilization, the samples showed no genetic toxicity (did not cause chromosomal aberrations) and no mutagenic effect, and did not increase the incidence of bone marrow cells micronucleus, demonstrating that the product material was quite stable after irradiation. Conclusions: No teratogenicity was induced in central vein catheters by the setting dose of irradiation sterilization. And therefore irradiation sterilization is a commendable method for such kind of materials, which deserves further promotion and application.

Abstract: Objective:To evaluate the blood compatibility of a central venous catheter in order to provide evidence for the safety of medical devices. Methods:The indexes of coagulation including thrombin time(TT), prothrombin time(PT), activated partial thromboplastin time (APTT) and fibrinogen (Fg) concentration, as well as the indexes of platelet function parameters including TXB2 and platelet count were measured, hemolysis test was conducted as well to detect the hemolysis rate of the sample. Results:There was no significant difference between the samples and negative controls on indexes of coagulation and platelet function (p>0.05), hemolysis test showed that the hemolysis rate of the sample was 1.35% (less than 5%), which was coincident with the criteria of the medical devices. Conclusion:The test sample of central venous catheter has good blood compatibility.

Abstract: To evaluate the blood and tissue compatibility of the dental implant samples, the whole blood clotting time, prothrombin time and partial prothrombin time, hemolytic rate and local implantation tests of the dental implant samples were determined, which will provide the basis for the clinical safety of such medical products. The WBCT, PT and PTT results showed that there were no significant difference (p> 0.05)between the testing groups and the negative control group, hemolysis rate of the dental implant samples was 1.38%, less than 5%, which in line with medical equipment blood compatibility test, all of these results indicated that the dental implant had a good blood and tissue compatibility.

Abstract: The aim of this study is to evaluate the blood compatibility of disposable burette transfusion apparatus and provide the basis for the clinical safety of medical products. Whole blood clotting time (WBCT), prothrombin time(PT),partial prothrombin time(PTT), hemolytic rate and bacterial endotoxin were measured. The results showed that there was no significant difference between the samples and the negative control on indexes of WBCT, PT and PTT (p>0.05), hemolysis test showed that the hemolysis rate of the sample was 1.38% (less than 5%), which was coincident with the criteria of the medical devices. Endotoxin levels were lower than 0. 5 EU/ml. All of the results above suggested that the disposable burette transfusion apparatus had a good blood compatibility and no pyrogen.

Abstract: Silver containing diamond like carbon films were coated on the surface of polyethylene film (PET) using novel hybrid sputtering method. Polymeric substrates can create soft, flexible, highly absorbent and cost-effective materials by selecting or controlling their molecular structures. The material silver is known to be a potential antibacterial material. The silver containing coating has been potentially recommended for synthesis biomedical materials. In the present work, we discussed the antibacterial activity of the silver containing DLC film coated PET film surfaces which was coated as a function of deposition power level. The surface morphology of the Ag-DLC was analysed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The atomic concentration and structure of the Ag-DLC was measured by energy dispersive X-ray (EDX) and Raman spectroscopy. The hydrophilicity of the films was measured by contact angle analysis. The antibacterial activity of Ag–DLC films were evaluated by bacterial eradication tests with Escherichia coli at incubation time of one day. In addition, blood compatibility of the Ag-DLC films were studied by In Vitro blood compatibility tests. It was found that the surface of the obtained Ag-DLC decreases with increasing the deposition power level. The antibacterial and hemocompatibility of the silver containing DLC film increase gradually with increase of deposition power level. Our results revealed that the Ag-incorporated DLC films are potentially useful as biomedical devices having good antibacterial and hemocompatibility.