Papers by Keyword: Bone Cell

Abstract: Aim of the study is to investigate the in vitro reaction between rat bone cell and poly (vinyl alcohol) (PVA)-matrix composites. Sample of Hydroxyapatite (HA) /PVA gel composites were prepared by the in situ growth method and Experiments of Alamar Blue Activity and Alkaline Phosphatase Assay were carefully made in our lab. SEM results displays that PVA-matrix composites containing HA fillers and pure PVA as a control group have an excellent biocompatible bone reaction in vitro with rat bone cells and HA filler particles in composites promote reaction between bone cell and composites. In vitro biology experiment resulted proves that adhesion, growth and differentiation of bone cells on all PVA-matrix composites are promoted by HA filler particles.

Abstract: Porous beads of bioactive ceramics such as HAp, TCP are considered to be promising as excellent scaffolds for cultivating bone cells. To realize this type of beads which maintains the function of scaffold with sufficient strength up to growth of new bone, and is expected to absorbed completely after the growth, a-TCP/ HAp functionally graded porous beads were fabricated. HAp is bioactive material which has both high strength and better tissue-adhesive properties, but that is not readily absorbed by the human body. On the contrary, a-TCP is highly bioabsorbable; it is quickly absorbed by the body, and, therefore, disappears before bone is completely replaced. Fabricated new beads are composed of a-TCP at the center and HAp at the surface, to control the solubility in living body. Bone cell adaptability of these beads were confirmed in vitro.