Papers by Keyword: Bone Regeneration

Abstract: Considering its functions (support, protection, assisting in movement and storage of minerals), the bone is an essential organ for the human body and the bone trauma/damages have a great impact on the human body functionality. For that reason a variety of biomaterials are studied for potential applications in bone regeneration or substitution. Bone substitution materials, with similar chemical composition to that of natural bone, and specifically those obtained by processes which mimic the natural bone formation in vivo, has been shown to be among the best. In this study, using a process of co-precipitation of calcium phosphate precursors on a mixture of biopolymers (chitosan, collagen, hialuronic acid) and magnetic nanoparticles (magnetite functionalized with chitosan), biodegradable biomimetic scaffolds have been obtained. In order to study their chemical structure, the biodegradable scaffolds have been characterized by Fourier Transform Infrared Spectroscopy (FTIR). The morphology of the biodegradable scaffolds, studied using scanning electron microscopy (SEM) indicated a macroporous morphology, which influenced the retention of simulated biological fluids. A direct relationship between the scaffolds’ degradation rate and the concentration of the polymeric phase has been observed. The in vitro cytocompatibility tests indicate that the prepared scaffolds are biocompatible and assure and adequate mediums for osteoblasts.

Abstract: Biodegradable scaffold is an accepted and commercialized medical alternative choice for bone regeneration. In this project, we used our new invention, porous starch-Hydroxyapatite (HA) composite for in vivo clinical trial. The products were prepared from medical grade Thai rice starch mixed with high purity (>97%) HA powder from fresh cow bone, and already passed in vivo animal biocompatibility test, then processed by freeze-drying. There were 44 volunteers from orthopedic and neurosurgical division, 4 and 40 patients, respectively. The results were assessed by operative surgeons and nurses, pre-and intraoperative period, including size appropriateness, comfort handle, ease of cutting, void space filling, water stability, product weight, shelf storage, package opening, contamination risk and waste removal. All average satisfactory scales were more than 95% rating. For postoperative period, at least 6 months, the soft tissue swellings around surgical areas were resoluted about 3 days as usual healing process. There were no any symptoms or signs of infection or allergic reactions. The follow up of x-ray imaging showed well ossification about 2 months. All patients have gained good functional performance. So porous starch-HA composites biomaterial can be used for human bone and skull regeneration with completely safety and efficacy.

Abstract: Aim of our preliminary in vivo study was to evaluate bone regeneration properties in three different bone conditions and to compare expression of OPG, NFkB105, BMP2/4, MMP2, IL 1 and IL 10 between healthy, osteoporotic and osteoporotic sham surgery affected rabbit bone controls. Osteoporosis index was found to be higher in healthy bone, while rabbits with osteoporotic sham surgery affected bone showed slight elevation of mean trabecular field rather than rabbits with osteoporotic bone. Expression of OPG and NFkB105 was higher in healthy bone, with less equal appearance in osteoporotic and osteoporotic sham surgery affected bone. Presence of BMP2/4 and MMP2 was much higher in healthy bone, whereas twice less in osteoporotic group. Osteoporotic sham surgery affected group showed recurrence of immunoreactive structures similar to healthy group of IL 1 and IL 10. OPG and was found as the most stable indicators for bone regeneration. Decrease of BMP2/4, MMP2, IL-1 and 1L-10 in osteoporotic bone with following increase in osteoporotic sham surgery affected bone, proves the role of traumatic injury as the inducer of bone regeneration.

Abstract: To target areas of the skeletal system which are clinically significant sites is a new strategy as the"local treatment of osteoporosis". Synthetic bioceramics implanted into critical sized bone defect of rats with experimental osteoporosis demonstrateds better effect to bone tissue repair in osteoporosis and/or osteoporosis status. Stem cell transplantation may improve bone mineral density in animal models of osteoporosis. An adequate blood supply of mesenchymal cells (MSCs and osteoprogenitors) is important for efficient bone regeneration. The concentration and quality of MSCs may vary significantly, depending on the individual (especially in older people), the cell obtaining sites and techniques used. Combination of BCP and stem cells are not studied on old experimental animals with double induced osteoporosis.

Abstract: Tympanoplasty is the reference for cholesteatoma treatment in ear, nose, and throat (ENT surgery), with the realization of a closing technique, which preserves the external auditory canal. An insufficient pneumatisation is an element favoring the occurrence and recurrence of cholesteatoma. In a prospective clinical pilot study, we have realized the reconstruction of the mastoid cortical bone by a Micro Macroporous Biphasic Calcium Phosphate bioceramics disks (MBCPTM Biomatlante France) using the closed technique, in order to maintain or increase the mastoid pneumatisation. Eleven patients were used for this study, and some of them have a sample collection for histomorphometry and histopathological analysis. Bone regeneration at the expense of the bioceramic was observed. The size and shape of the disk was not preserved due to the large resorption and bone ingrowth at the expense of the implant. However 6 patients presented cholesteatoma recurrence. The MBCPTM disks are able to reconstruct the mastoid cortical and maintain the mastoid pneumatisation contrarily to the technique of filling the mastoid cavity. However, the high rate of cholesteatoma recurrence observed in our study, confirmed the interest to fill mastoid that suppress airspace posterior cavities.

Abstract: Silica-calcium-phosphate composite (SCPC) is a drug delivery platform that has successfully demonstrated the ability to bind and release several therapeutics including antibiotics, peptides, anticancer drugs, and growth factors. It has successfully demonstrated a unique capacity for bone regeneration. The present studies address the effect of the phosphate and silicate functional groups on drug binding and controlled release kinetics of Cisplatin (Cis). Moreover, the roles of ceramic composition and resorbability on rhBMP2 release kinetics and bone regeneration in a critical size calvarial defect in rabbit is presented.

Abstract: Bio-absorbable materials have been strongly needed in bone regenerative surgery. β-TCP ceramics have been widely used as bone tissue scaffold materials, due to their bio-compatibility and bio-degradation. The aims of this study are to estimate blood permeation into different porous β-TCP blocks (75% and 67% in porosity), and to evaluate the behaviors of the 75% porous β-TCP block in rat subcutaneous tissue and sheep iliac bone defect by histological observation and 3-dimensional (3D) imaging analysis by μ-CT. The 75% β-TCP block revealed better performance in blood permeation than the 67% β-TCP in a dish including 3ml of sheep blood at 2 and 10 minutes. Almost area of the 75% β-TCP block turned to red at 10 minutes. In rat subcutaneous tissue, the bulk region of the 75% β-TCP was stained with HE. TRAP-positive multinucleated giant cells appeared on the surface of bulk at 4 weeks. In sheep iliac bone defect (10×15×9 mm³) model, μ-CT showed bone ingrowth into almost pores of the 75% β-TCP block at 2 months, and the block was absorbed and replaced by new bone until 4 months. The block was reduced to one-third in horizontal length and from 10 mm to 4 mm in vertical length at 2 months by 3D images. Body fluid stained by HE was found in the bulk region. We believe the body fluid permeation inside the bulk of the 75% porous β-TCP should contribute to the initial cell adhesion, proliferation and differentiation, and its biodegradation. It was concluded that the super porous β-TCP block with hydrophilic property might be a biological scaffold, harmonized with bone remodeling.

Abstract: There are numerous clinical indications for bone grafts. The ideal graft material should favor bone apposition and growth while simultaneously being degraded by body fluids and cells. Ultimately, the material should be replaced by mature bone tissue within a healing period of weeks. Because autologous and allogenic bone grafts fulfill some of these requirements, these biological materials are routinely used by clinicians. However, biological materials have intrinsic limitations. Harvesting autologous bone requires a second surgical site, which can cause complications, the material is limited in quantity, and it may lead to immunogenic rejection or transfer certain pathogens and viruses [1-3]. For these reasons, researchers and clinicians have developed synthetic bone substitutes. Our approach has focused on composite biomaterials that combine bioceramics with hydrogels to replace and regenerate bone tissue in osseous defects.

Abstract: The aim of this study is to assess the histological efficiency of low level laser therapy (LLLT) with respect to the acceleration of bone regeneration after surgical treatment of intrabony defects. Twenty patients with intrabony defects, aged between 20-45, non-smokers, good health condition present at the time of the surgery, were randomly divided in two groups, control, ten parients, and test group, ten patients. Informed consent was obtained. Each patient presented at least one periodontal defect treated by bone allograft. The test group received postsurgical treatment with low lever laser therapy (LLLT). The equipment used was OsseoPulse AM300, at an intensity of 20mW/cm², for 20 minutes per day, for 21 consecutive days. The control group received no treatment with LLLT. The bone formation was evaluated in both groups at baseline and 6 months postoperative by the means of tissue biopsy followed by a histological analysis. The histological study of the test samples at 6 months after regeneration showed bone formation without inflammatory cells and occasional nonviable bone consistent with regenerating bone. No evidence of the grafted material was present. The histological study suggests that in 6 months there was new bone formation in the defects treated with LLLT. Clinical data indicate the possibility of more rapid wound closure and subsequent healing in zones treated with LLLT as compared with control.

Abstract: This study evaluates the effect of two novel particulate silicon-doped calcium phosphate graft materials as compared to the currently clinically used material β-TCP on osteogenesis and bone formation after implantation in critical-size defects the sheep scapula. These materials were developed in order to create biodegradable bone substitute materials that degrade rapidly, but still stimulate osteogenesis at the same time, thereby resulting in bone repair and regeneration with fully functional bone tissue. All bone substitute materials studied facilitated excellent bony regeneration of critical-size defects in the sheep scapula. Of the three grafting materials studied, the calcium alkali orthophosphate material with the crystalline phase Ca₂KNa (PO₄)₂, with a small amorphous portion containing magnesium potassium phosphate and a small addition of sodium magnesium silicate had the greatest stimulatory effect on bone formation and expression of osteogenic markers, while exhibiting the highest biodegradability.