Papers by Keyword: Carboxymethyl-Chitosan

Abstract: The clarify fruit juice has the characteristics of clear bright and current commercially available in the beverage industry and international trade market. Chitosan clarification method has the compatibility, complete degradation and degradation metabolites of low toxicity and good biological and is widely used in juice production in the fruit and vegetable clarification. Some research shows that factors affecting the results of juice clarification are chitosan dosage, temperature, time, solution of pH, the molecular weight of chitosan solution viscosity size.Chitosan used in fruit juice processing could clarify fruit juice, but also had the characteristics of gel in the juice stored, and could maintain the original freshness of fruit juice. Bentonite, zeolite, maifan stone,such as the load with chitosan applied to fruit juice clarification, the amount of random factors difficulty to control can be avoided. Caroxymethyl chitosan used in blackberry juice processing the content of protein, polyphenol in juice reduced more than the chitosan treatment.It was good to prevent turbidity during storage or secondary precipitation.

Abstract: Natural bone is a composite mainly made from nano/micro-structure of hydroxyapatite and collagen fibers. For bone regeneration by tissue engineering, it is important to synthesize nano-composites with good biocompatibility, high bioactivity and great bonding property as potentially useful scaffold. In this study, we fabricated chitosan nano-nonwoven scaffold via electrospinning and modified chitosan scaffolds by carboxymethylation (CM). Moreover scaffolds were macerated in SBF (simulated body fluid) to form hydroxyapatite on its surface. Surface morphologies (SEM) showed that nano/micro particles formed on the surface of the carboxymethyl chitosan fibrous scaffold. Results of FT-IR and XRD confirmed that the nano/micro particles were hydroxyapatite crystalline. Moreover by employed mice osteoblast (MC3T3-E1) cell for adhesion, proliferation and differentiation assays, and the hydroxyapatite particles appeared to have a great effect on the late stages of osteoblast behavior (alkaline phosphatase ).

Abstract: In this study, it is shown that it is possible to prepare carboxymethyl-chitosan/Ca-P hybrids using an innovative “auto-catalytic” co-precipitation method, namely by using an acid and an oxidant bath. The X-ray diffraction (XRD) patterns evidenced the formation of crystalline calcium-phosphate precipitates when using an acid bath, while amorphous ones were obtained for those produced in the oxidant bath. The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM/EDS) studies revealed that the extent of the polymer precipitation and formation of calcium-phosphates is directly dependent on the pH and composition of the baths. Furthermore, by conducting bioactivity tests in a simulated body fluid (SBF) followed by the SEM/EDS analysis it was possible to detect the formation of an apatite layer with a cauliflower-like morphology on the surface of hybrids prepared by the acid bath, after 7 days of immersion. These results are quite promising because they can allow for the production of bioactive and biodegradable 3D porous scaffolds to be used in bone tissue engineering applications.