Papers by Keyword: Chitosan (CS)

Abstract: Tissue engineering is the promising technique to replace organ transplant in order to solve many problems regarding the shortage of organ donor and other problems related to organ or tissue transplantation such as bleeding, diseases, accidents and others. Polycaprolactone (PCL) and chitosan (Cs) are biodegradable polymers that have the properties to develop into scaffold. Both of the polymers were used in this research in order to produce scaffold by using freeze drying technique. Four samples of scaffolds were fabricated with the variety of concentration chitosan, PCL 10% (1g of PCL in 10ml glacial acetic acid), PCL/Cs 11% (1g of PCL and 0.1g of chitosan in 10ml glacial acetic acid), PCL/Cs 12% (1g of PCL and 0.2g of chitosan in 10ml glacial acetic acid) and also PCL/Cs 13% (1g of PCL and 0.3g of chitosan in 10ml glacial acetic acid). The produced scaffolds had good properties from both polymers such as faster degradation rate, hydrophilic, porous and others.

Abstract: Scaffolds-based tissues engineering involves the combination of an artificial extracellular matrix (ECM), living cells, with high porosity and well connected pores that will provide suitable environment for cells. In this study, firstly, poly (caprolactone) (PCL)-based microspheres were synthesized and characterized. Bovine serum albumin (BSA) (0.04% w/v) was added into the microspheres produced from 5% (w/v) PCL concentration. BSA loaded microspheres were then incorporated into chitosan solution to fabricate porous scaffolds. The scaffolds were then characterized using different techniques.

Abstract: Recently, in pharmaceutical research and industry scientists widely have used polysaccharides and other cationic polymer which is one of the most extensive studies in the field of non-viral DNA carriers for gene delivery and therapy. As a purpose of present study variations of the final solution pH values and filtration were examined for their effects on the particle size and the tendency of particle formation. Chitosan nanoparticles were prepared based on the ionic gelatin of chitosan which hydroxyapatite will adsorb onto the chitosan nanoparticles to form complexes of chitosan and hydroxyapatite. The resulting nanoparticles had a size and positive electrical charge, which vary depending on the formulation conditions. The physicochemical properties of the nanoparticles were determined by scanning electron microscope (SEM). Besides of that, element and chemical characterization of samples were assessed by Energy-dispersive X-ray spectroscopy (EDX). The data revealed that the chitosan/DNA nanoparticles were successfully prepared with a nanosize range. Obtained complexes could be loaded by variants of DNA for further use in gene delivery applications.

Abstract: The artificial neural networks (ANNs) non-modeling methods were selected to optimize the preparation of loading norcantharidin chitosan nanoparticles (NPs) by ionic cross-linkage. A multiple regression model was constructed for fitting several preparation factors and each of the factor level values was arranged in the L9(34) design table and their linear weighted sum of the normalized value was taken as optimized object. A Back-Propagation (BP) network (3×7×2) in ANNs was created and trained for further checking the optimal results and the trained network was applied to simulate the experiment system and screen the optimal conditions. Finally, the best condition was obtained.

Abstract: The invention of plastic film has brought not only convenience but also environmental pollution to people. To use natural vegetable fiber is the mainly approach to solve the problem. In this paper, the waste cotton fiber was used as raw material and the cotton nonwoven sample was made through wet laying process, after strengthened by chitosan, degradable film was prepared. The performances of the film were characterized and analyzed by testing the strength, air permeability, moisture permeability and other indexes. The results showed that chitosan can be used as strengthening agent, the performances of the film strengthened were improved, and it was degradable.

Abstract: The controlled-release tablets of sasanquasaponin (SQS) were prepared by using SQS, cornstalk and chitosan as the main drug and accessories. The effect of the particle size of cornstalk on release rate was studied. The thermal stability and wet stability of the controlled-release tablets of SQS were investigated. The controlled-release tablet of SQS was characterized by IR techniques. The releasing rate of the controlled-release tablets of SQS are controlled by controlling the particle size of cornstalk. The thermal stability and wet stability of the controlled-release tablets of SQS are good. The chemical bonds are formed among SQS, cornstalk and chitosan.

Abstract: The present study evaluated the properties of the polymeric blend films obtained from chitosan and corn starch using glutaraldehyde as crosslinking agent and glycerol as plasticizer by the casting/solvent evaporation method. Fourier transform infrared (FTIR) analyses confirmed that the groups interactions of starch and chitosan in starch-chitosan blend films were present especially when using glutaraldehyde as crosslinking agent. Differential scanning calorimetry (DSC) studies revealed an endothermic peak of starch film at 98 oC corresponding to water evaporation. DSC also indicated that the endothermic peak of blend films moved to higher temperature with adding chitosan and glutaraldehyde compared with native corn starch film. The tensile strength of the blend films increased with the increasing content of glutaraldehyde. The starch-chitosan blend films exhibited the highest tensile strength of 11 MPa when the content of starch was 9% (w/v in water), glutaraldehyde content was 1.0 mL, chitosan ratio is 0.35 (w_chitosan/w_starch), glycerol ratio was 0.35 (w_glycerol /w_starch).

Abstract: With the improvement of living standards, the requirement of food quality, nutrition, and freshness have been higher and higher. As the characteristics of highly effective, non-toxic, low cost, easy operation and significantly preservation effect, chitosan has been used widely as a new type of natural antistaling agent. Chitosan fresh-keeping mechanism and application in food preservatives are discussed in this study. From the three aspects of chitosan coating, composite coating, and chitosan modification, this work provides an overview of some researches about chitosan fresh food technology in abroad and domestic. And further development is discussed: the effect of the sole use of chitosan is not very good. Composite coating of chitosan will be more and more attractive with the merits about effectively preventing moisture loss, reducing weightlessness rate and so on. Storage stability about liquid chitosan can be solved by modified chitosan, which will also have a more attractive effect. This paper has theoretical significance to develop chitosan applications in food fresh-keeping technology.

Abstract: Chitosan is a new kind of material in life-science, it can be used in lipid-lowering. Chitosan microspheres (CTS-MP) were prepared by spray-drying, chitosan nanoparticles (CTS-NP) and chitosan-capsaicin microspheres (CTS-CAP-MP) were prepared by ionic gelation process. The effect of these samples on lowering lipids was evaluated by measuring its’ binding capacities with lipids under the conditions mimicking the gastrointestinal tract in vitro. Also, the particle sizes of chitosan preparations were examined by scanning electron microscopy (SEM) and particle size analyzer. The results showed that the lipids-binding capacities of CTS-MP and CTS-NP were far more higher than that of CTS and CTS-CAP-MP. In conclusion, CTS-MP and CTS-NP will be better fat-lowing food additives to replace chitosan. The mechanism of lipids-binding in vitro is not only physical adsorption. Particle size is not the determinant factor, electrostatic action is maybe another factor of lipids-binding.

Abstract: Magnetic microspheres with chitosan (CTS) and nano-iron (NI) as materials were synthesized by reverse suspension polymerization technology, and then modified with ethylenediamine (EDA). The average amine content of as-prepared magnetic microspheres (CTS-NI-MM-EDA) is 5.22 mmol/g, and the iron content is 23.77%.The adsorption experiments indicated that the adsorption rate of CTS-NI-MM-EDA for methyl orange is in the range of 62%-86% when the concentration of methyl orange is less than 250 mg/L, and the saturated adsorption quantity is 1651mg/g at 25 oC and pH 5.5.