Papers by Keyword: Confocal Laser Scanning Microscope

Abstract: In this paper, a new Rockwell hardness model with volumetric parameter by least square method was suggested. Also, its result was compared and investigated with real measured hardness. The volume measurement of indented volume was performed by CLSM(confocal laser scanning microscope) and the captured HEI(height encoded image) was used as a original data for the calculation of indented volume. Using this volume as a parameter, this independent variable was used for experimental modeling of least square algorithm and it can be used for the prediction of Rockwell hardness.

Abstract: Quantitative surface measurement is an important field in many applications including materials science and engineering quality control. A non-destructive and versatile technique for quantifying surface roughness in 3D is Confocal Laser Scanning Microscopy (CLSM). However, this technique has not been widely accepted to use for quantitative surface measurements due to limited work on it. The project has researched the suitability of using the system for surface characterization and appropriate settings for image acquisition for quantitative surface analysis. Based on the above fundamental work, this study has developed and presented a comprehensive approach of using the system for quantitative surface characterization through image processing, 3D image construction, image stitching and numerical image analysis. The surface characterization results presented in the paper have demonstrated that the system can be used to accurately measure surface roughness of engineering surfaces.

Abstract: Current anatomical theory does not recognize the existence of an extended floating threadlike structure inside the blood vessels. Nonetheless, this study developed a new method for observing such an intravascular threadlike structure. The key technique involves injecting acridineorange into the femoral vein to circulate along the blood vessels and stain the nuclei of the intravascular threads inside the blood vessels. In-situ observations were then made under a fluorescence stereomicroscope after saline-perfusion. Confocal microscope images revealed a distinctive characteristic pattern of nucleus distribution that was clearly distinguishable from fibrin, capillaries, small venules, arterioles, or lymph vessels. Accordingly, it is suggested that the identified intravascular threads are part of the Bonghan's circulatory network that is distributed throughout the body, including inside the blood vessels.