Papers by Keyword: Culture Media

Abstract: As an industrial organic solid waste, oil residue, with wide sources and low price, but could not find an effective way to use currently. In addition, with the development of mushroom industry and rising price of traditional raw-materials, it is very necessary to find the substitute raw material to reduce production cost of mushroom cultivation. This study attempts to produce the strains of Flammulina velutipes by taking use of oil residue to replace part of the traditional raw material, there are two groups in the experiment: the test group is adding 5%, 10%, 15%, 20%, 40% of the oil residue to replace the culture medium of cottonseed hull and wheat bran; the control group is the traditional culture medium, then comparatively observed the growth of the mycelium. The results showed that it can promote the growth of the mycelium and shorten the cycle of seed production to add the oil residue in the test. But the growth rate of mycelium will be hindered when the concentration exceeds a certain range.The optimal culture medium to add the oil residue is as follows: 15% of the oil residue, 64% cottonseed hull, 20% coarse wood chips, 0% wheat bran , 1% lime. Oil residue contains a lot of the nutritional components to benefit the growth of edible fungi, and without the heavy metal pollution, can replace the culture medium of cottonseed hull and wheat bran and other traditional materials, reduce cost of production the strains, a fair-sized economic efficiency can be received, the environment can be protected.

Abstract: Isolation and culture of hydrogen-producing and fermentative bacteria is an important foundation on biohydrogen production process. There are complicated operation and composition in present anaerobic techniques and culture media. Hungate technique was improved and plate of culture bottle was established by comparing anaerobic methods and bacterium growth. Isolation and enrichment culture media were confirmed by the test of different composition and the species and amount of hydrogen-producing and fermentative bacterium. 550 bacterium strains were isolated by the anaerobic operation.

Abstract: Photosynthetic bacteria (PSB) have various applications but the culture cost is very expensive. To find an efficient and economic culture medium the effects of yeast extract, C/N ratio, and trace elements on the growth of PSB were studied. The results showed that the optimal condition for PSB growth was: yeast extract of 100mg/l, C/N of 12:1, and trace elements (in mol/L): Mn2 + (0.009), Fe3+ (0.0025), Co2+ (0.0024), Cu2 + (0.0024), and Zn2 + (0.0033). Trace element lack could affect the growth of PSB. The order was Mn2 +> Fe3 +> Co2+> Cu2 +> Zn2 +. The improved medium was named HCH, and the optimum medium components were (in g/l) DL-malic acid: (4), MgSO4: (0.12), (NH4)2 SO4: (1), CaCl2: (0.075), KH2PO4: (0.5), K2HPO4: (0.3), Na2EDTA: (0.02), yeast extract : (0.1), trace elements 1ml (in mol/l ): Fe3+: (0.0025), Mn2+: (0.009), Zn2+: (0.0033), Co2+: (0.0024), pH:6.8. Comparing with the traditional RCVBN medium, in HCH medium yield of PSB increased 1.2 times and the cost decreased 19.8 times.

Abstract: The coatings formed on the H2O2-treated titanium substrate by electrodeposition were used in order to evaluate the difference of transformations in the simulated body fluid (SBF) and the culture medium with MG63 cells. A porous hydroxyapatite (HA) coating with relatively low crystallinity and large crystallites was formed on the H2O2-treated titanium substrate by electrodeposition. HA coating transformed for 5 days in the SBF consisted of densely-packed rod-shaped crystallites with various differentiated grains. Octacalcium phosphate (OCP) and HA coating transformed for 5 days in the culture medium consisted of both flake-shaped and rod-shaped crystallites with indistinct grains. MG-63 cells were well attached and proliferated during the transformation into this flaked-shaped OCP. This difference between transformations of the HA coatings in the acellular SBF and in the culture medium with MG63 cells is due to different ion composition in each solution and proteins in culture medium.