Papers by Keyword: Cytotoxicity

Abstract: The increasing demand for effective ocular prosthetics has led to the exploration of innovative materials for orbital implants. The study focuses on the preliminary biocompatibility assessment of nanozinc bioceramic orbital implant intended for anophthalmic socket applications. These implants aim to address common complications such as infection and inflammation in patients requiring ocular prostheses. Bioceramic orbital implants were fabricated using conventional techniques using biphasic calcium phosphates and kaolin clay as raw materials. The bioceramic material, engineered for its antibacterial properties, is evaluated for its cytotoxic response. In vitro tests are conducted to determine the cellular response and antibacterial efficacy of the implants. Effect of different loadings of nanozinc oxide onto the bioceramic orbital implant were investigated. Disc diffusion method using test organisms Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) has revealed the resistance of the bioceramic orbital implant against bacterial attack. Moreover, MTT cytotoxicity test has shown fibroblast cell viability indicating good biocompatibility. Ultimately, the bioceramic orbital implant has presented no adverse effects upon exposure to Albino rabbits using dermal and eye irritation tests. The findings from this preliminary research will provide crucial insights into the feasibility and safety of using antibacterial bioceramic materials for orbital implants, potentially improving clinical outcomes for patients with anophthalmic sockets.

Abstract: Two potential novels Schiff base iron (Fe) complexes Fe-L² and Fe-L³ (where L²= N, N'-bis (o-hydroxyacetophenone) ethylenediamine and L³= N, N'-bis (o-hydroxybenzaldehyde) phenylenediamine) were synthesized from interaction a hot methanolic solution of each ligand L² or L³ (0.01mole) with the appropriate amount of Fe (NO₃)₃.9H₂O metal salt (0.01mole). This study investigated the cytotoxicity induced by both complexes (0.1 to 100 µg/ml) in MCF-7 and MDA-MB 231 cell lines. After 24 hours of treatment, the cell viabilities of both MCF-7 and MDA-MB-231 cells were linearly proportional to the Fe-L² concentrations. A higher concentration of Fe-L² would cause higher cell killings. On the other hand, most of the Fe-L³ concentrations caused total cell deaths for both cell lines, except for the lowest concentration (0.1 µg/ml). Fe-L² and Fe-L³ also caused lower cell viability of MDA-MB-231 cells compared to MCF-7 cells. Overall, the obtained Fe-L³ is more toxic than Fe-L² in breast cancer cells. It is suggested that the Fe-L³ is an excellent agent against breast cancer cells; meanwhile, the Fe-L² is biocompatible and a good support in medical applications, especially as a radiosensitizer in radiotherapy.

Abstract: Chlorhexidine gluconate mouthwash has a poor taste during and after rinsing which makes its users dissatisfied. Therefore, the product must be improved to have a better taste and still retain its effectiveness against oral pathogens. To evaluate in vitro antimicrobial activity and toxicity effects of Newly formulated chlorhexidine mouthwash. The antimicrobial activity of the mouthwash was evaluated by Agar well diffusion method against the tested microorganisms. The toxicity test was performed by using the MTT assay. The new formula has the potential to treat and prevent oral and throat infections. The newly developed Chlorhexidine mouthwash can be considered safe for oral usage.

Abstract: Antibiotic-resistant has emerged without new drug challenges. Polymyxin B (PMB) was the last resort therapy for multiple-drug resistant Gram-negative bacteria. However, the toxicity of PMB including nephrotoxicity (61%) and neurotoxicity (7%) was dose-limitation. PMB-based sodium deoxycholate sulfate (SDCS) formulations were prepared in the 2-different mole ratios of SDCS to PMB (5:1 and 10:1). Particle size, zeta-potential, and drug content were evaluated. The biocompatibility of PMB formulations was investigated with normal human primary renal proximal tubule epithelial cells (PCS-400-010), human kidney epithelial cell lines (HEK 293T/17), human kidney cell lines (WT 9-12), macrophage-like cells (RAW 264.7) and red blood cells (RBC). PMB formulations had smaller particle sizes and lower zeta-potential when compared to PMB. PMB content presented from 97-100% after lyophilization. PMB-SDCS formulations revealed lower toxicity to cell lines than PMB, especially SDCS: PMB (5:1) and low lysis of RBC. PMB-SDCS mixture had better biocompatibility than those PMB and SDCS alone.

Abstract: Maxillofacial prosthesis is a non-invasive procedure to replace damaged or lost facial tissue structures. Extraoral maxillofacial prosthesis to resemble facial skin tissue is made of silicone. The addition of fillers and pigments to silicone has been done to improve its physical and mechanical properties while maintaining its biocompatibility. This study aims to evaluate the cytotoxicity of nano silica filler and intrinsic pigment additives in clean grade silicone as an alternative material for maxillofacial prosthesis. Thirty clean grade silicone samples were divided into 6 treatment groups: 1 group of silicone without any additives and 5 groups of silicone with addition of intrinsic pigment and nano silica filler with concentration of 0%, 1%, 2%, 3% and 6%. Cytotoxicity was assessed by MTT assay against 3T3L1 fibroblasts. The average absorbance value for all treatment groups was 0,941-1,105, which was lower than the control group. The post hoc test showed that only the 6% of nano silica and intrinsic pigment additive group did not differ from the control group. The addition of silica nano filler and intrinsic pigment to silicone did not cause toxicity to 3T3L1 cells. The cytotoxicity of nano silica is determined by its particle size and concentration. The use of intrinsic pigments produces compounds that are less reactive to genetic material. In conclusion, the addition of nano silica filler and intrinsic pigment to clean grade silicone as an alternative maxillofacial prosthesis is nontoxic. The addition of 6% nano silica filler and intrinsic pigment to clean grade silicone showed the best cell viability.

Abstract: Ti-6Al-4V is one of the popular choices for biomedical implants due to multiple advantages, such as corrosion resistance, high strength-to-weight ratio, biocompatibility, lightweight, durability, and osseointegration properties. However, Young’s modulus (E) of Ti-6Al-4V is much higher than the E of natural human bone, which may lead to stress shielding. Therefore, it is critical that we need to fabricate the implant with specific mechanical properties that can match the patient’s existing bone. With the advent of 3D printing, we now can design porous structures with the most suitable E through adjusting porosity to suit individual needs. Porous structures with various porosities were manufactured by selective laser melting (SLM). Mechanical testing was performed to show that the E of the printed samples was related to the porosities only. Based on the simulated and actual results, there are still many areas that can be improved to enhance the quality of the printed structures. Indirect cytotoxicity tests were performed to verify the biocompatibility of the porous structures.

Abstract: Alot of medical and industrial applications used the metal nanoparticles (NPs) with increase interest to be used as cancer therapy. The current work aimed to prepare AuNPs and AgNPs through the use of plasma jet and test their antitumor mechanism of apoptosis induction. The results indicating the face-centered cubic structures and crystalline nature of AuNPs and AgNPs. Also, the image of FESEM showed that the well dispersions regarding AuNPs and AgNPs, while the NP’s spherical shape with the particle size distributions which are considered to be close that estimated from the XRD. cytotoxicity have been assessed against the Normal embryonic cell line REF and the digestive system (HC , SK-GT-4) cell lines under a variety of the series dilute of the Ag and Au NPs (6.25, 12.5, 25, 50 and 100%), have been determined through a microtetrazolium (MTT) assay. The capacity of Ag and Au NPs to induce apoptosis to an infected cell has been studied by crystal violet stain to measure the percentage of induction of apoptosis. In cases where 100 μg\ml Au NP concentrations are 69.60 percent, the maximum cytotoxicity of the HC cell line was reported, while 100 μg\ml Au NP was 69.20% for the SKg cell line exposure. qRT-PCR in AuNPs and AgNPs treated of (HC and SKG) cell lines revealed a remarkable in the expression of BAX, BCL2 and AIF, Endo G (independent pathway).

Abstract: Numerous studies have shown titanium dioxide nanoparticles (TiO₂ NPs) could present a risk or potential risk to humans and other living organisms in certain conditions via inhalation and skin contact. Dermal exposure has limited adverse effects and the possible risks for exogenous inhaled nanoparticles migrating to the brain through the olfactory nerve is still under research. To study the in vivo and in vitro neurotoxicity of brain tissue in rats induced by TiO₂ NPs. For in vitro study, rat astrocytes were exposed to TiO₂ NPs with three different diameters (10, 50 and 200 nm) at five concentrations levels. Cellular morphology and sulfur rhodamine B (SRB) were carried out to evaluate the viability of particle-treated cells after 72 hours exposure. For in vivo study, suspensions of test material above mentioned were injected into tracheas of Wistar rats at dose of 0.1, 1.0 and 10.0 mg·kg-1 in three groups, respectively. After 72 hours of exposure, the concentration of TiO₂ NPs in brain tissue and the levels of IL-1β, TNF-α and IL-10 in brain homogenate were measured, while the cell morphology induced by TiO₂ NPs was observed by light microscopy and transmission electron microscopy. TiO₂ NPs can significantly affect the growth and morphology of rat astrocytes and inhibit the proliferation of astrocytes, which was positively related to dose-effect and size-dependent response. Pathological observations indicated that TiO₂ NPs could penetrate the blood-brain barrier, leading to blood-brain barrier damage in rats, brain tissue necrosis, mitochondrial swelling and apoptosis while the non-nanoscale TiO₂ particles showed no significant toxicity in the central nervous system cells.

Abstract: Daily walking or exercise of the bone implant recipients may generate particles due to wear and tear. Reports have mentioned that particles could circulate in the human body and trigger aseptic loosening, inflammation, and other potential complications. The mechanism of these phenomena remains mostly unclear. This study is to investigate the cytotoxicity of titanium (Ti), stainless steel 316 (SS316), and magnesium (Mg) particles due to these materials are the most commonly used biomaterials based on their adequate mechanical properties and excellent biocompatibility. Human osteoblasts (SAOS2 cells) were exposed directly to different concentrations of Ti/SS316/Mg particle during the direct cytotoxicity test. Together with the previous study, we found out that Ti particles showed cytotoxicity to osteoblasts at different dosages and times, while SS316 particles and Mg particles (low dosage) can reduce the cytotoxicity induced by Ti particles and boost cell viability. Mg particles can be toxic to osteoblast at a higher dosage, while SS316 particles are “safer” than Mg particles at higher dosages. Cell viability and cell morphology of SAOS2 cells under different treatments were observed at 2/3/5 days. This study found out that cell viability could be enhanced with certain combinations of Ti/SS316/Mg particles. This can give us certain guideline on how to design and fabricate a hybrid bone implant. However, how to quantify the particles inside the human body in real-time, and the exact interaction among particles, cells, tissues, and even organs require further research.

Abstract: Modeling of the relationship between structure and cytotoxicity has helped in the process of designing safer new drug compounds. In this study, modeling was carried out between the structures of 29 betulinic acid derivatives with their cytotoxicity. The modeling is done by using multiple linear regression (MLR) techniques. In the model, an equation is obtained by involving five descriptors and has statistical parameters as r²_training of 0.776; F_cal/F_tab of 4.503; r²_test of 0.985; r²_m of 0.971. The five descriptors involved in the equation are TDB2e (3D topological distance-based autocorrelation-lag 2/weighted by Sanderson electronegativities), TDB9s (3D topological distance-based autocorrelation-lag 9/weighted by I-state), RDF50m (radial distribution function-050/weighted by relative mass), RDF140m (radial distribution function-140/weighted by relative mass), and RDF10s (radial distribution function-010/weighted by relative I-state). The equation could be used to design the new betulinic acid derivatives with lower predicted cytotoxicities regarding the coefficients of the descriptors. In this case, the new substituent is chosen to enhance the value of RDF140m and RDF10s, while also to make the value of TDB23, TDB9s, and RDF50m getting lower, so the CC₅₀ value will rise (the compound become less toxic to the normal cell).