Papers by Keyword: DBP

Abstract: In our research the influence of thermal heating on the release of plasticizers from plastics into the meat product and water was researched. The two most widely used plasticizers, dibutyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP), were detected. Both of the plasticizers have a negative effect on the health. And both are regulated by regulation of the European Union. In this study were researched phthalates in products prepared by sous-vide technology and it was studied effect of reheating on migration of phthalates. The average concentrations of both phthalate esters, DBP and DEHP, in heated water decreased. The average concentration of DBP in water before heating was detected 0.82 mg/100 ml and after heating 0.52 mg/100 ml, the average concentration of DEHP in water before heating was established 0.33 mg/100 ml and after heating 0.19 mg/100 ml. In plastic wraps, average DBP concentration with reheating increased and average DEHP concentration with reheating dropped. The average DBP concentration in plastic packaging, heating at 60 °C/12 h was determined of 9.87 mg/dm² after reheating 60 °C/1 h was detected of 15.40 mg/dm². The average concentration of DEHP in plastic packaging, heating at 60 °C/12 h was determined of 58.71 mg/dm² and after reheating 60 °C/1 h was detected of 28.28 mg/dm². The average DBP concentration in meat products due to reheating decreased, the average DEHP concentration in meat products increased. The average DBP concentration in meat products heating at 60 °C/12 h was detected of 8.39 mg/g dry matter and after reheating of 6.43 mg/g dry matter, the average DEHP concentration heating at 60 °C/12 h was determined of 25.37 mg/g dry matter and after reheating of 65.51 mg/g dry matter..

Abstract: Dibutyl phthalate (DBP), an accepted environmental endocrine disrupting compound, which has anti-androgenic effects,is widely used in many industries such as plastics, pharmaceuticals, plastic food packaging materials. This article intends to study the effect on JNK/MAPK pathways in the damage of rat testicle sertoli cells induced by DBP via vitro test. Testicular sertoli cells of rates were incubated by medium containing DBP in different concentrations (0μg/ml,1μg/ml,10μg/ml, 100μg/ml). after culturing for 24 hours the expression of vimentin was detected by immunofluorescence; the expression of vimentin mRNA detected by RT-PCR; Western blot analyzed the expression of related protein of JNK/MAPK pathways in sertoli cells. The results showed that vimentin highly express in sertoli cells and gradually decrease with the increase of poisonous doses. The mRNA expression of vimentin in each exposure group has a downtrend with the increase of does. The total protein of JNK and P-JNK protein increased gradually in a dose effect manner. These results suggested that DBP can decrease the expression of vimentin mRNA, the decrease of which was regulated by JNK/MAPK signal transduction pathway that activated by DBP, and then induced the apoptosis of Sertoli cell.

Abstract: Dibutyl phthalate (DBP) as a commonly used plasticizer can be detected in many environmental medium. In this study oxidative damage effect on testicular germ cells and liver cells in mice induced by DBP were investigated via vitro test. Testicular germ cells and liver cells of mice were incubated with medium containing DBP in different concentrations (0μmol/L,10μmol/ L,50μmol/L,100μmol/L), after one hour thiobarbituric acid assay and xanthine oxidase assay were used to measure the levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) respectively.The results showed that the levels of MDA in testicular germ cells and liver cells increased gradually with the increase of DBP concentrations, while compared with the control group, the activities of SOD declined significantly at dosage of 50μmol/L and 100μmol/L. These results suggested that DBP could cause lipid peroxidation in testicular germ cells and liver cells of mice in vitro. Oxidative damage may be one of its toxic mechanisms. It would provide an experimental foundation for security application of DBP.

Abstract: Many different types of liquids and materials are used during service of motor vehicle, for example fuels (petrol, diesel, LPG, CNG), lubricants (oils, plastic lubricants), brake fluids and cooling fluids, cosmetics, etc. Vehicles are made from different construction materials also. Especially it can be steels, glass, lights metals, textile, leather, plastics, thermoplastics, etc. These operational and constructional materials must satisfy many requirements from the view of function which are demanded for example additional stability of construction of cars, minimal weight, heat removal (cooling fluids), transfer of braking power (brake fluids), optimizing of friction but they have to be researched from the view of health and ecology hazard. Materials which interiors are made from often release volatile and partly volatile substances. With the regard on human health, equipments of cars should be made from materials which release chemicals at a minimum level, which hasn't negative impact on quality of air in a closed space. Health impacts could increase in case when substances react together, although the intensity of releasing of these substances is decreasing during first weeks. For the improvement of elasticity chemical materials plasticizer based on the esters of acid phthalate (PAE) are used. The PAE are a big hazard for human health. Exposition of population can be by inhalation, oral, and by skin-resorption. Ten samples of new operation materials for vehicles have been examined for the detection of the presence of acid esters (PAE). The results show that almost in all investigated samples the presence of phthalate acid esters was proved. Phthalates are animal carcinogens which are capable to cause necrosis or malformation of tissues. They are dangerous for liver action and they can cause the reproduction toxicity of laboratory animals. Neither the legislation of the Czech Republic nor EU deals with such problems.

Abstract: Phthalate esters (PAEs) have been widely added as the plasticizer to increase the flexibility and ductility of polymeric materials. In this study, organic solvents, such as methanol, hexane, ethyl ether and acetone, were used for the direct extraction of plasticizers in neoprene, nitrile, and PVC gloves, under a temperature range of 20-80°C. The results showed that DEHP (Di-2-ethylhexyl phthalate) and DBP (Di-n-butyl phthalate) were analyzed in all three glove samples. The extraction capability of PAEs from the PVC gloves was the highest, while that from the neoprene gloves was the lowest. It was found that the extraction capabilities of DEHP from the glove samples were proportional to Log K_ow of the extraction solvents. This result implied that PAEs were more soluble in a non-polar solvent and likely to be extracted from the glove samples. In addition, the amount of extracted PAEs increased with the experimental temperatures. As workers wearing the chemical protective gloves, there is a need to understand the dissolution potential of PAEs in the gloves and to assess the skin exposure of PAEs.

Abstract: The toxic effects of di-2-ethylhexyl phthalate（DEHP）and di-n-butyl phthalate（DBP）on the kidney of mouse was studied. Forty mice were randomly divided into 4 groups , 10 mice in each group. There were three experimental groups:DBP+DEHP（2.5g/kgDEHP+0.5g/kgDBP）,DEHP(2.5g/kg）,DBP（0.5g/kg）and a control group which was given coin oil. The experiment was conducted through gavage every other day for 35 days. The organ coefficients were counted and pathological changes in kidney were observed. The weights of mice of each group increased with the time,but there were no difference between each group in statistic. The organ coefficients of kidney of DEHP and DBP&DEHP group were significantly higher than that of DBP group and control group. Infected groups appeared injury to some extend by observing the pathological section. It reveals DBP and DEHP have toxicity on kidney.

Abstract: Because use of plastic in cotton field made a lot of dibutyl phthalate contaminants, they were introduced to the soil of cotton field. Through the germination of cotton seeds and cotton seedling physiological indicators will be measured to study the dibutyl phthalate on cotton growth and development impact. The results showed that different concentrations of n-butyl phthalate had some of the cotton seed germination inhibition, and with increasing concentrations its inhibition increased .But at low concentrations of dibutyl phthalate promoted seed germination. Dibutyl phthalate produced an effect on cotton SOD, POD and CAT activity, with increasing the concentration of DBP, SOD and CAT activity of cotton leaves began to increase subsequently reduce the phenomenon appears. MDA levels have been rising, cotton cell membrane damaged.

Abstract: This paper studied the estrogenicity of six typical aqueous EDCs by examining their proliferation of MCF-7. These six EDCs were 17-β Estradio (E2), biphenol A (BPA), nonyl phenol (NP), di-n-butyl phthalate (DBP), hexachlorobenzene (HCB) and Cadmium (Cd). Results showed that MCF-7 proliferation was sensitive to these compounds. E2 was the most sensitive one and the effective concentration was 10^-14 mol/L, while Cd was the least sensitive one and the effective concentration was 10^-8 mol/L. The sensitivity of these six EDCs ranked as: E2 > BPA ≥ NP > DBP ≥ HCB > Cd. On the other hand, the cell proliferation extent of these compounds ranked as: HCB > DBP > BPA > Cd > NP ≥ E2. Very complex dose-time-response curves were observed for all six EDCs, indicating that many factors impacted the cell grow.

Abstract: Organic synthesis chemistry is a very fast-growing discipline and it plays a very important role in chemistry. With the development of organic synthetic chemistry, organic synthesis device has also made greater development. At the same time, advances of organic synthesis device further promote the development of organic synthetic chemistry. This paper describes the working principle and system architecture of organic synthesis device and takes a case of di-n-butyl phthalate (DBP). At last, I foresee great prospect for organic synthesis

Abstract: This study was designed to investigate the influence of demineralized bone particles (DBP)/PLGA hybrid scaffold on angiogenesis and osteogenesis in a calvarial defect model. DBP/PLGA scaffolds were manufactured by solvent casting/salt leaching method, and each scaffold contained 0, 10, 20, 40, and 80 wt% DBP of PLGA, respectively. A total of 34 rats were operated and bicortical holes were placed on their calvaria. The defects were filled with different ratio DBP/PLGA scaffolds. After 3, 7, 14, and 28 days, specimens were taken and, histologic, immunohistologic and RT-PCR analyses were carried out concerning number of vessels and density of regenerated bone, and angiogenic activation. On days 7, in all experimental groups, bone formation occurred in a direction from defected margin of calvarium to center of implanted scaffold and new vessel formation took place in front of the osteogenic regeneration front. We found that the 20 and 40 wt% DBP/PLGA scaffold was superior in its ability to regenerate new bone, induced more intensive formation of microvasculature and expressed in a higher level of osteocalcin mRNA than other groups.