Papers by Keyword: Dental Pulp Stem Cells

Abstract: One of the main reasons for limiting the widespread clinical use of mesenchymal stem cells (MSCs) is a low speed of their proliferation in vitro. In this regard, the search for new safe and effective growth stimulants is an urgent task. In this study, we investigated the effect of nanocrystalline cerium oxide doped with gadolinium (Ce_1-х Gd_х O_y), on the morphofunctional characteristics and proliferative activity of MSCs derived from dental pulp. It was shown that the introduction of Ce_1-х Gd_х O_y nanoparticles into the culture of dental MSCs provides the activation of proliferation of the cells in a dose-dependent manner. High concentrations of Ce_1-х Gd_х O_y nanoparticles inhibit the proliferation of the cells; however, this does not lead to further development of apoptosis and cell death. The obtained results indicate that the nanocrystalline cerium oxide can be considered as a basis for the development of highly efficient and low-cost supplements for culturing MSCs.

Abstract: Dental pulp cell research might open a promising application in tooth tissue regeneration. The aim of this study is to establish a protocol for in vitro culture the human dental pulp stem cells to apply in tissue engineering. Human premolar and impacted third molars were collected and disinfected. Dental pulp fragments were cultured with Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) medium supplemented with 10% Fetal Bovine Serum (FBS). Dental pulp stem cells (DPSCs) were identified using proliferation assay, RT-PCR and flow cytometry. Growth of DPSCs on dentin surface was assessed by MTT assay. The study showed that we successfully isolated, cultured and characterized dental pulp cells by outgrowth method. Cultured population of cells expressed in high level of Oct4, CD146, CD90, CD44. DPSCs proliferated on chemically and mechanically treated dentin surface. This research provides important information and a basis for further investigations to establish dental tissue engineering protocols.