Papers by Keyword: Electrophoresis

Abstract: Polyaniline (PANI) was prepared by chemical oxidation of aniline for formation of stable colloidal suspension. The suspension was prepared by dispersion of PANI/HCOOH stock solution into acetonitrile. The stable suspension of positively charged PANI colloids was deposited by applying electrophoretic technique on the surface of copper sheet. The coatings obtained were adherent to the copper substrate. The effect of operating parameters such as the deposition time, the concentration of solids in the colloidal suspension, and the voltage applied were examined on the deposition efficiency. The efficacy of the coated layer in corrosion resistance was evaluated using potentiodynamic (Tafel) polarization with a scan rate of-500 to +500 mV. The annealed PANI coated layer showed the highest corrosion efficiency of 86.5% followed by the normal PANI coated layer with inhibition efficiency of 84.4%. The mean roughness and the 3-D morphology of the obtained coated layers from the Atomic Force Microscopy (AFM) were used to explain the surface energy of the coated layers. The rms values obtained for bare copper, annealed PANI coated layer, and the normal PANI coated layer were 63 nm, 182 nm, and 254 nm respectively. The characteristics of the coated surface were examined using Scanning Electron Microscopy (SEM) which indicated a porous layer on the surface of the copper. The crystallographic structure of the coated layers were examined using XRD.

Abstract: This paper describes the development and operation of a small parts coating device in an electrophoretic manner. The cataphoretic coating process is nowadays highly desirable and generates a continuous coating against corrosion. The aim of the research is to find solutions or innovations to coat small parts in bulk. Small parts are inserted inside the device and painted in a cataphoretic manner. Painting is based on rotary device and random contact between small parts and cathode.

Abstract: Three procedures for protein extraction in longan pulp had been applied to analyze protein pattern and quality of Longan pulp (Dimocarpus longan Lour. cv. Daw) during fruit growth to increase protein expression in proteomic analysis at Maejo university’s farm. There were data points to compare between normal and physiological disorder syndromes during fruit growth (5,10, 15, 20, 25 and 30 weeks, respectively) by one dimensional electrophoresis (1-D gel) technique in reducing condition. The first protein extraction, M1 (95% ethanol) showed obviously 15 protein bands which molecular weights were 14.97, 17.90, 18.30, 21.63, 28.54, 31, 33.96, 35.02, 42, 51.69, 65.69, 71.54, 88.02, 106.86 and 130 kDa, respectively. While M2 extraction (phenol-methanol/ammonium acetate) and M3 extraction (1.5 mM tris-HCl pH 8.0, 5 mM EDTA, 2% SDS) had low protein expression and no sharpness (13 and 12 protein bands, respectively). In different extraction conditions, therefore, M1 was a suitable method because of highest protein bands and obvious protein expression on longan pulp for proteomic analysis. Proteomic analysis of M1 extraction method was used in protein analysis by using LC-MS / MS techniques. It was found that the heat shock protein 83 (81.0 kDa), a family of proteins that was produced by cells in response to exposure on stressful conditions, the elongation factor 1-alpha (49.45 kDa), a selective regulator of translation, and the peroxidase 4 (39.74 kDa), a protein that is involved in the degeneration or aging of cells. These proteins exhibited a darker appearance of the protein bands at 30 weeks. Moreover, a partial glyceraldehyde-3-phosphate dehydrogenase (34.06 kDa), the protein involved in metabolic processes in glucose degradation, was also founded a darker appearance at 25 weeks and low appearance at 30 weeks of abnormal longan. However, higher proteomic techniques should be studied to confirm this biomarker protein in the further.

Abstract: Proteomic investigation of leaves, peels and seeds on longan (Dimocarpus longan Lour. cv. Daw) at Maejo University’s farm in Chiang Mai province, Thailand. There were comparisons between normal and physiological disorder syndromes in longan on fruit growth (5, 10, 15, 20, 25 and 30 weeks fruition, respectively) by one dimensional electrophoresis (1-D gel) technique at a concentration of 12.5% gel in reducing condition with Coomassie brilliant blue R-250 staining coupling on liquid chromatography tandem mass spectrometry (LC-MS/MS). The research showed that vegetative storage protein (25.2 KDa), a storage protein in plants, was expressed in leaves, peels and seeds on before and after physiological disorder syndrome occurring. A protein involved on photosynthesis, the ribulose-1,5-bisphosphate carboxylase/oxygenase (48.3 KDa), was represented only on leaves at 30 weeks after fruition but not in peels and seeds of longan. Two proteins, the BnaC01g20210 (35.8 KDa) and predicted protein (40.11 KDa), which could not be identified the type and function of the metabolism, were decreased in diseased longan. These proteins may be important protein in part of the recovery process of abnormal longan. Therefore, advanced technique will be used for further proteomic studies.

Abstract: Detergents represent a unique class of chemical compounds. They can alter surface and interphase bonds, and form micellar systems. These detergent properties allow to alter wettability of surfaces, stabilize or destabilize dispersed systems, and modify the properties of liquid phases. Therefore, the use of detergents is virtually unlimited in chemical synthesis and processing, medicine, biological systems and agricultural biology. The article includes the studies of the feasibility of application of 1,1,1,3,5,5,5-heptamethyltrisiloxane modified by polyalkylene oxide in combination with allyloxypolyethyleneglycol in the ratio of 10:1 as a detergent for agrobacterial-mediated transformation. Tween 20 detergent was used as a means of control in the concentration of 5%. As a result of histochemical analysis of transformed tissues, a significant difference was determined in expression of beta-glucuronidase reporter gene. The study of gene expression by calculating the relative content of mRNA showed that the initial number of mRNA copies of genetic makers transfected by co-culturing in a liquid medium with addition of Tween 20 is on the average 32% lower than when using modified heptamethyltrisiloxane

Abstract: Electrophoretic deposition of ligand-free platinum nanoparticles has been studied to elucidate how wettability, indicated by contact angle measurements, is linked to vital parameters of the electrophoretic deposition process. These parameters, namely the colloid concentration, electric field strength and deposition time, have been systematically varied in order to determine their influence on the contact angle. Additionally, scanning electron microscopy has been used to confirm the homogeneity of the achieved coatings.

Abstract: Cathode samples of titanium-based nanodiamond field emission were prepared by electrophoresis dispersed solutions in different formulas. Observe the surface and structure of the coating by using metaloscope, microscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD); Test the field emission characteristic after annealing; Analyze the relation between the samples’ light photos and the uniformity of coating; Discuss the mechanism of the improved field emission characteristic after annealing. Under the same conditions the surface uniformity of diamond coating prepared by isobutyl alcohol is best,it is the minimum threshold electric field emission, 5.5 V/μm, the current density can reach to 85μA/cm² at the field of 15V/μm. Field emission characteristic and luminescence could be best after annealing.

Abstract: The purpose of this paper is to investigate the electrophoretic deposited nanosilica on stainless steel for corrosion protection. The electrophoretic deposition (EPD) was carried out by pulsed direct current (PDC) of silica sol made of sodium silicate. The amplitude and frequency of the PDC were varied in the range of 0.1-0.6 V and 30-80 Hz, respectively. The corrosion protection properties of the silica films were analyzed by Electrochemical Impedance Spectroscopy (EIS) in 2 wt% NaCl solution. The experimental results showed that the silica films exhibit good characteristics as indicated by their high pore resistance and low admittance. The silica film corrosion protection characteristics were influenced by the frequency and amplitude of PDC during preparation of electrophoretic deposition.

Abstract: The tissue samples of cisplatin-resistant and sensitive patients with osteosarcoma were obtained through chemosensitivity testing before. The typical one of cisplatin-resistant and sensitive osteosarcoma tissue samples were analyzed by two-dimensional electrophoresis and the relevant differentially expressed proteins were obtained. The sequences of differentially expressed proteins were obtained through the analysis of mass spectrometry technology to be identified comparing with protein databases. It is concluded that there are differentially expressed proteins in tissue of cisplatin-resistant and sensitive osteosarcoma.

Abstract: In the present study, the Acetyl-coenzyme A carboxylase (ACCase) of Chlorella sp. USTB-01 purified and characterized. Molecular weight of ACCase was calculated to be about 456 kD and was comprised of two identical subunits of 229 kD. The enzymatic characteristics isoelectric point was 6.7. Moreover, a rapid and sensitive assay of High performance liquid chromatography (HPLC) was established to measure overall activity of ACCase in low protein content condition by detect the ACCase activity by measuring ADP production and Acetyl-coenzyme A (Acetyl-CoA).