Papers by Keyword: Enrofloxacin

Abstract: This research aims to investigate synthesis parameters for modification of chitosan with enrofloxacin. Most modifications of chitosan are focused on amino group. There are many methods for modification of chitosan, for example, conjugation of amino group with carboxylic group by coupling agents. 1-Ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride (WSC) and N-hydroxysuccinimide (NHS) are widely used coupling agents for amide formation under mild conditions. Those two coupling agents were applied in this study. Optimum amount and ratio of coupling agents, amount of enrofloxacin and reaction times were investigated. The chemical structures were characterized by Fourier transform infrared spectroscopy (FT-IR). The optimum synthesis conditions were determined from the absorbance ratios. Chitosan-enrofloxacin has antibacterial activity against both S. aureus and E. coli.

Abstract: Extracellular polymeric substances (EPS) in marine algae- Platymonas subcordiformis affected by enrofloxacin (ENR), the typical quinolone antibiotics released to the environment through different ways, was studied. A 3-dimension EEM (excitation –emission matrix) fluorescence spectroscopy was used to examine the interaction between EPS and ENR, and the fluorescent peaks A and B were detected, whose fluorescence intensity remarkably decreased with the increased ENR concentration. It was demonstrated that the interaction of EPS with ENR well fitted the modified Stern-Volmer. It was concluded that the presence of EPS in marine algae affected the transport and transform of antibiotics in aquatic environment.

Abstract: To generate a group-specific monoclonal antibody (McAb) against danofloxacin (DANO), enrofloxacin (ENR) and ciprofloxacin (CIP), glutaraldehyde was used to link the presentative hapten of CIP to the immunogen and coating antigen, respectively, leaving the common structure of these 3 drugs exposed in both conjugates as a major antigenic site. Consequently, a McAb (6D3) with high cross-reactivity to this three antibiotics has been obtained by using hybridomas technique. In a biotin-avidin mediated enzyme-linked immunosorbent assay, the IC₅₀ values for DANO, ENR and CIP were 5.1, 4.5 and 4.2 ng mL^-1, respectively. The ELISA was used for the detection of spiked DANO and ENR+CIP in milk. The recoveries ranged from 74.1 to 92.4% and coefficients of variation were in a range of 6.6-11.9%. The accuracies and sensitivity of the method were good for simultaneous analysis of the 3 drugs in milk after a simple sample extraction process.

Abstract: The photochemical degradation of two antibiotics, enrofloxacin and ciprofloxacin hydrochloride, was preliminarily studied under irradiation with a high-pressure mercury lamp (HMPL) in this paper. The influence of initial concentration of antibiotics, Fe (Ⅲ) and platymonas subcordiformis on the degradation efficiency of antibiotics were investigated. The results suggested that in the presence of P. subcordiformis, the degradation efficiency of enrofloxacin and ciprofloxacin hydrochloride were 85.0% and 84.2% in aqueous solutions underwent photodegradation under HMPL irradiation, respectively. Moreover, the lower initial concentration of antibiotics improved photodegradation rate of antibiotics.

Abstract: A rapid indirect competitive ELISA (icELISA) for the determination of enrofloxacin (ENR) residue has been developed. EDC method was employed to synthesize the artificial antigen of ENR-BSA, and anti-serum produced from rabbits was selected. Based on the square matrix titration, an icELISA method was developed with the polyclonal antibody. The Linear range was from 0.006 to 31.5 ng/mL, with LOD and IC50 values of 0.003 ng/mL and 0.45 ng/mL, respectively. Except for a high cross-reactivity (105.2%) to ciprofloxacin, negligible cross-reactivity to other compounds was observed. The recoveries of ENR were in the range of 98.3-127.5%, 85.7-112.5% and 97.4-103.8% for chicken muscle, liver and kidney, respectively. Therefore, this assay has the potential to be incorporated into a quantitative monitoring program for the rapid screening of ENR residue in food.

Abstract: This article aimed to optimize a polyclonal antibody based indirect competitive ELISA (icELISA) and compare between the icELSIA method and the LC-MS technology for the determination of enrofloxacin (ENR) residue. Based on the square matrix titration, linear range of the icELISA was from 0.006 to 31.5 ng/mL, with LOD and IC50 values of 0.003 ng/mL and 0.45 ng/mL, respectively. After optimization, 0.03 mol/L of HCl was used in the assay buffer and this ELISA system can tolerate acetonitrile not higher than 10%. The recoveries of ENR were in the range of 98.3-127.5%, 85.7-112.5% and 97.4-103.8% for chicken muscle, liver and kidney. The correlation coefficients (R2) of the ELISA and LC-MS data were 0.9472 in muscle, 0.9843 in liver, and 0.9382 in kidney, respectively.

Abstract: A rapid indirect competitive ELISA (icELISA) format has been developed for the determination of enrofloxacin (ENR) residues in chicken. For this purpose, carbodiimide active ester method was employed to synthesize the artificial antigen of ENR-BSA, and anti- serum produced from the immunized rabbits was tested by indirect ELISA and icELISA. By the square matrix titration, the icELISA method was developed for the quantitative detection of ENR, based on the pAb. The Linear range was from 0.006 to 31.5 ng/mL, with LOD and IC50 value of 0.003 ng/mL and 0.45 ng/mL, respectively. Except for a high cross-reactivity (105.2%) to Ciprofloxacin, negligible cross-reactivity to the other compounds was observed. The recoveries of ENR were in the range of 98.3-127.5%, 85.7-112.5% and 97.4-103.8% for chicken muscle, liver and kidney, respectively. After optimization, 0.03 mol/L of HCl was used in the assay buffer and this ELISA system can tolerate acetonitrile not higher than 10%. The correlation coefficients (R2) of the ELISA and LC-MS data were 0.9472 in muscle, 0.9843 in liver, and 0.9382 in kidney. Therefore, this assay has the potential to be incorporated into a quantitative monitoring program for the rapid screening of ENR residue in food.

Abstract: This paper presents the generation of monoclonal antibodies (mAbs) with high specificity against enrofloxacin (ENR) through cell fusion procedures, and the development of a mAb-based indirect competitive ELISA (icELISA) method to detect ENR residue using one of these Hybridomas (clone 4B5-D6). Under the optimal experimental conditions, this assay exhibited a working range of 0.004-38 ng/mL with IC50 and LOD values of 0.4 and 0.002 ng/mL, respectively. Except for a high cross-reactivity (105.2%) to Ciprofloxacin, negligible cross-reactivity to the other compounds was observed. After optimization, 10% of methanol was used in the assay buffer and this ELISA system can tolerate acetonitrile not higher than 10%. Recovery studies indicate that an excellent correlation between concentration spiked and concentration determined was found, and the results also suggest this assay has the potential to be incorporated into a quantitative monitoring program for the rapid screening of ENR residue in poultry tissues.