Papers by Keyword: Ferritin

Abstract: A ferritin mutant A1C with 24 free SH groups on its outer surface was prepared, which was successfully cross-linked to hen eggshell membrane outer layer (ESM-OL) by a disulfide linkage as characterized by SEM and TEM. Generally, this ferritin conjugated ESM-OL (Ft-ESM-OL) exhibited a significantly higher activity of heavy metal ion uptake as compared to ESM-OL alone under the same experimental conditions. For example, the adsorption capacity of Ft-ESM-OL for U⁶⁺ and Fe²⁺ were 21.16% and 18.4% higher than that of natural ESM-OL, respectively. On the other hand, the recovery capacity of Fe²⁺ by Ft-ESM-OL was 8.11 mg/g ESM, a value 67.9% higher than that of natural ESM-OL. Thus, this newly ferritin-modified ESM-OL greatly improved the adsorption and recovery efficiency of heavy metal ion as compared to ESM-OL alone.

Abstract: Prussian blue nanoparticles (PBNPs) have been certified a kind of mimetic enzyme possesses the advantages of stability, high catalytic activity and low prices. Ferritins are natural nanoscale structures with unique three-dimensional structures and biological functions. In this context, we synthesized PBNPs on the surface of the iron oxide core of ferritin taking use of the ferric iron of the core, we also intended to retain the specificity of ferritin for some biological use. Our results show the resulting nanostructures (Prussian blue-ferritin nanoparticles, PB-Ft NPs) got very small size and relatively high catalytic activity , furthermore, PB-Ft NPs successfully combined the intrinsic enzyme mimetic activity of PBNPs and the specificity to tumor cells of ferritin. The peroxidase-like activity and catalase-like activity of PB-Ft NPs were studied. Peroxidase-like activity which fits well the Michaelis-Menten kinetics was found strongly depending on pH, temperature and the concentration of PB-Ft NPs. Then a sensitive method for glucose detection was developed using glucose oxidase (GOx) and PB-Ft NPs. PB-Ft NPs displayed catalase-like activity in PH higher than 5.0, the generated oxygen was measured by the dissolved oxygen electrode.Enzyme-linked immunosorbent assay (ELISA) shows PB-Ft NPs possess both specificity and peroxidase-like activity.

Abstract: Novel assemblies of gold nanoparticles or gold nanoparticles with other inorganic nanoparticles have received considerable interest due to their fascinating properties desired for various detection applications. Here we present a bioconjugate containing ferritin and gold nanoparticles. In this bioconjugate, there is a central ferritin, and a several gold nanoparticles are located around by DNA base-pairing.