Papers by Keyword: Fibroblast

Abstract: Jasminum sambac (J. sambac) has been used as a medicinal herb with wound healing property. This experimental research proposed to evaluate the effect of J. sambac flower extracts on fibroblast cell viability and film characteristics which maybe benefit in development of wound dressing film. The testing extract of J. sambac flowers were prepared as filtration extract (F), compression of the residue flower extract (C), combination of filtration and compression extract (FC) and ethanolic extract (E). The testing extracts were evaluated the effect on fibroblast cell viability using resazurin reduction assay. NIH 3T3 fibroblast cells were treated with variable concentration of J. sambac extracts for 24, 48 and 72 h. prior to evaluate cell viability. The results demonstrated that the F and FC extract of J. sambac flower at concentration of 31.25-500 µg/ml did not induce cytotoxicity on NIH 3T3 fibroblast cells given non-cytotoxic effect. The cell viability seemed to increase at 48 h of treatment with concentration of 31.25-250 µg/ml. Meanwhile, C and E extract induced cytotoxicity on NIH 3T3 cells by reduction of cell viability. The F and FC non-cytotoxic extracts were selected to examine ability of film formation without film-based compounds and assessed physical and mechanical characteristics of the prepared films. The experimental results revealed F and FC extracts could form film after incubation at 60°C. The better physical and mechanical characteristics of the films was presented in FC film which the prepared film revealed smooth surface and better absorption and elongation. Furthermore, gelatin was blended to FC extract for assess improvement of film properties. The results revealed that gelatin-blended FC extract film gave better mechanical characteristics of the film. Non-cytotoxicity of the FC extract of J. sambac flowers on fibroblast cells and appropriate physical and mechanical characteristics of FC film may point to the usefulness of J. sambac flower extracts in herb-based film for wound dressing.

Abstract: Graphene is a single-to-few layer carbon sheet, consisting of carbon atoms hybridizing with one another via sp² configuration, providing outstanding mechanical, electrical, and electrochemical properties that can be utilized in various applications. In this work, we synthesized graphene powder via a modified Hummers method to obtain electrically insulating graphene oxide (GO), and converted the GO to semiconducting reduced graphene oxide (rGO) using L-ascorbic acid as a reducing agent. The rGO was re-dispersed in DI water and cast on prefabricated electrodes to create an rGO film chemiresistive sensor.The rGO sensor was shown to detect changes in buffer pH and cell concentrations of three different cells: human breast cancer cells (MDA-MB-231); non-small-cell lung cancer cells (A549); and fibroblast cells (L929). Sensor performances were determined in terms of "sensitivity", ratio of normalized resistance change upon exposure to a certain analyte concentration, and the analyte concentration. Our studies serve as strong evidence that the rGO-based chemiresistive sensor can be used for a quick and easy test in indicating buffer pH, or quantifying concentrations of a known cell.

Abstract: During electrospinning process, charged web jets solidify with solvent evaporation and form two-dimensional (2D) web-like nanofibers on the collecting plate. In this paper, Poly [(lactic acid)-co-(glycolic acid)] (PLGA) solutions are used to produce different 2D web-like nanofibers, which can mimic the physical features of natural extracellular matrix (ECM) at the nanoscale and improve fibroblast growth. According to mass conservation, the possible mechanisms for the web formation are investigated.

Abstract: NiTi alloy is one of important materials in orthodontics. Shape memory effect of this material ensures the possibility to deliver highly desireable light forces for thooth movement. Unfortunately, NiTi has problem in the high amount content of Ni that can cause allergy or even risk of poisioning in the human body due to Ni ion released. To overcome this problem, several methods of coatings were performed to prevent the releasing of Ni ion. They were Electropolishing Pretreatment combined with Photo Electrocatalytic Oxidation, TiN Pack Cementite, Advanced Oxidation Process and Biomimetic Hidroxyapatite. The objective of this research was to analyze the effect of various coatings to the biocompatibility of orthodontic wire. Biocompatibility of coated NiTi wire was investigated through MTT assay using BHK-21 fibroblast cell in order to analyze the toxicity of each coating method. In addition, the surface roughness was determined using Atomic Force Microscope (AFM). Furthermore, the attachment and spreading of fibroblast cell were observed by Scanning Electron Microscope (SEM). The result was revealed that biomimetic HA coating has the highest cell viability percentage due to its surface morphology.

Abstract: Silk fibroin/ propylene glycol blend films were prepared by mixing silk fibroin and propylene glycol (PG) at different ratios. X-ray diffraction and infrared spectroscopy were used to investigate the structure of the blend films, and the results showed that with the content of PG increasing, the structure of the blend films was silkⅠcrystalline structure and then was gradually changed into the crystalline structure of Silk II. The dissolution loss rate was greatly improved, and the transmittance was still kept in good degree. Besides, the blend films had best flexibility when the weight of PG/ SF was 3/10. The fibroblast was culviated on it to evaluate its biocompatibility. The results showed that the blend films could support the attachment and growth of flbroblast cells as a substratum.

Abstract: FGF-2-apatite and FGF-2-zinc-apatite composite layers were formed on commercially available anodically oxidized Ti external fixation rods using FGF-2-and ZnCl₂-containing supersaturated calcium phosphate solutions. The FGF-2-zinc-apatite composite layers precipitated on the Ti external fixation rods significantly enhanced proliferation of fibroblastic NIH3T3 and osteoblastic MC3T3-E1 cells in vitro.

Abstract: Tetrandrine can inhibit the proliferation and collagen synthesis of fibroblasts in lung and liver tissue confirmed by a series of clinical research. In this chapter, we investigated the effect of Tetrandrine on the proliferation of human dermal fibroblasts derived from hypertrophic scars. The dermal fibroblasts were isolated from human hypertrophic scar tissues and cultured in vitro. Tetrandrine with different concentration were added to culture medium respectively. The proliferative activities were determined. The result show that when the concentration of added Tetrandrine increased from 5μg/ml to 80μg/ml, the proliferative activities of cultured dermal fibroblasts were decreased gradually in dose-dependent manner. It conclusions that Tetrandrine can obviously inhibit the proliferation of human dermal fibroblasts derived from hypertrophic scars.

Abstract: The cementum, a mineralized tissue lining the tooth root surface, is required for development of a functional periodontal ligament. The presence of healthy cementum is considered to be an important criterion for predictable restoration of periodontal tissues lost as a consequence of disease. Despite the importance of cementum to general oral health, very little is known about the cells responsible for the formation of cementum, cementoblasts. The aim of this study was to examine the effect of the ionic products from the dissolution of bioactive glass with 60% of silica ( BG60S ) on the behavior of cementoblasts, osteoblasts and fibroblasts. The cell viability was tested by MTT assay based on mitochondria activity of the cell and Trypan Blue assay based on membrane cell viability. The membrane cell viability measured by Trypan Blue assay showed the beneficial effect on all the cell types tested. It was observed a higher proliferation in the presence of ionic products from dissolution of BG60S when compared to control. In the MTT assay we also observed increased cell viability on all the cell types, but proliferation of cementoblasts was higher (107%) than observed for the other cells (104%) compared to control. The results from this study suggest that Cementoblasts, osteoblasts and fibroblasts are important cells on events that control the development of mineralizing and not mineralizing tissues and the investigation of the comparative behavior of these cells can be a useful experimental model. The observed effect of the bioactive glass particles on cementoblasts shows that this material is an interesting alternative to be used in composite membranes for cementum tissue engineering.

Abstract: Poly-L-Lactide (PLLA) composites were prepared with nano silver particles with the weight ratios of nano silver particles to PLLA (Ag/PLLA) at 0.5%, 2.5%, 5%, 7.5% and 10% (w/w). In vitro cytotoxicity tests were conducted firstly to evaluate the cytotoxicity of the composites. The results indicated that PLLA, 0.5%, 2.5% and 5% Ag/PLLA composites were nontoxic to cells, while 7.5% and 10% Ag/PLLA composites were significantly toxic to cells. Cell proliferation experiments and antibacterial tests were also performed. 5% Ag/PLLA was found the best for cell proliferation with obvious antibacterial property. Thus, Ag/PLLA can be used as nontoxic scaffolds for tissue engineering with antibacterial property.

Abstract: Glucans have been reported to stimulate immunity and to promote wound healing. Adult human dermal fibroblast (aHDF) cultured in serum free (serum-starvation). Proliferation of aHDF was measured at various concentrations of β-glucan by MTT assay, and migration was observed for 36h on microscope. The result of fibroblast bioassay, β-glucan had positive influence. In this study, the direct effects of β-glucan on proliferation and migration of human dermal fibroblasts were examined in vitro. That means β-D-glucan has the effect to enhance proliferation and aHDF migration speed, and has the potential as a wound healing agent.