Papers by Keyword: Glucose Oxidase

Abstract: Diabetes mellitus (DM) is a chronic metabolic disease with an increasing prevalence. Conventional treatments, such as insulin injections, often result in unstable blood glucose levels. This study explores a glucose-responsive drug delivery system based on Zeolitic Imidazolate Framework-8 (ZIF-8) integrated with Glucose Oxidase (GOx) and gold nanoparticles (AuNP) for Type 2 Diabetes Mellitus (DM-2) treatment. The combination of pH-sensitive ZIF-8 with glucose-responsive GOx aims to regulate glucose fluctuations via controlled drug release. Characterization results show that synthesized ZIF-8 maintains its structure and morphology, even after the integration of metformin, GOx, and AuNP, confirmed by X-Ray Diffraction (XRD), Fourier Transform Infra-Red (FT-IR), and Field Emission Scanning Electron Microscopy (FE-SEM) analyses. In vivo testing demonstrated that Met-GOx@ZIF8/AuNP effectively stabilizes blood glucose levels in diabetic mice, indicating its high potential as a glucose-responsive DM-2 therapy. In vivo experiments showed that metformin encapsulated in ZIF-8 with GOx and AuNP significantly improved glycemic control compared to conventional treatment. This system offers a promising solution for patients with busy lifestyles by providing a controlled, glucose-responsive drug release.

Abstract: Biosensor is a device that detects any change in physical parameter of biological elements in physicochemical manner and transfers its output in electrical manner. Nanotechnology is playing an emerging role in the development of biosensors and carbon nanotube is the leading one in this developing field. Carbon nanotube has an excellent combination of mechanical, electrical and electrochemical properties that has stimulated increasing interest in the application of CNTs as component in biosensors. This paper provides a review of analytical modeling of biosensors on the basis of carbon nanotube.

Abstract: Enzymes including starch-degrading enzymes and glucose oxidase are used in one-bath process to desize and bleach cotton fabrics. The effect of enzyme concentration on pretreatment efficiency, especially bleaching of cotton fabrics has been investigated by innovatively focusing on glucose conversion ratio of desizing products. The results showed that the starch sizing materials of cotton fabrics were completely removed by starch-degrading enzymes. The conversion ratio of glucose in desized liquor increased with concentration of glucoamylase. Consequently, whiteness of cotton fabrics bleached in one-bath process was also improved, and an increase of 7% in whiteness of bleached fabrics was observed. The fabric tear strength was decreased. However, the loss ratio could be controlled below 15%. The properties of softness and adsorption of bleached cotton fabrics were satisfactory.

Abstract: Glucose biosensor has been improvised from time to time in order to provide fast and accurate detection of glucose concentration especially for diabetic patients. Recently, nanoparticles have rising attention engaging with biosensor application. Nanoparticles serve as the carrier of enzyme immobilization whereas; enzyme is a biological catalyst that reacts with specific substrate in metabolic reaction. Glucose oxidase (GOx) in particular, is the most common enzyme used for fabrication of biosensor. Glucose measurement was done by using amperometric measurement that converted corresponding biochemical reaction between glucose and GOx into electrical output. Response behavior studies were conducted in order to compare the successfulness of GOx immobilization onto GOx-biosensor. Immobilization of glucose oxidase onto nanoparticles can lead towards tremendous impacts especially in new, high sensitivity biosensor.

Abstract: Conducting poly (3, 4-ethylenedioxy-thiophene) (PEDOT) thin film was prepared onto gold electrode by electrodepositing from aqueous electrolyte. Glucose oxidase (Gox) was further immobilized on the PEDOT/Au by physical adsorption. The morphology the modified electrode was investigated by FE-SEM. In phosphate buffer medium (0.1mol·L^-1 pH 5.5), cyclic voltmetric results indicated that GOx can be immobilized on the PEDOT film, and the biological activation of GOx was retained. A direct electron transfer was found between immobilized GOx and electrode, which suggested that the PEDOT film could not only supply a platform for enzyme immobilization but also offer a effectively electrical contact with the active sites of enzyme.

Abstract: This work aims to obtain hybrid from nanoparticles of Fe₃O₄ modified surface with 3-aminopropyltriethoxysilane (APTES) for their use in the immobilization of glucose oxidase (GOX). The obtained material was characterized by XRD, FTIR, SEM, sedimentation test and magnetic separation. Based on the obtained results it showed that the surface modification with APTES was successfully achieved and that the APTES did not changed the magnetic characteristic neither the structure of Fe₃O₄, indicating that the hybrid material obtained is promising for immobilization of GOX.

Abstract: Glucose oxidase (GOD) (EC 1.1.3.4) is an oxido-reductase that catalyses the oxidation action of glucose to hydrogen peroxide and D-glucono-δ-lactone [. GOD is widely used in the determination of free glucose in body fluids, in vegetal raw material and in the food industry [. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies. Free GOD will be denatured and inactivated rapidly due to its structural instability under extreme pH or temperature [. Four immobilizing methods, including physical adsorption, covalent binding, crosslinking and embedding methods, were used to improve their economic feasibility. Covalent binding combined active functional group monomers of carriers onto enzymes [4-. Researches showed that oxirane groups can react with-NH₂ and-HS of enzymes under mild conditions so that the enzyme molecules were immobilized on the copolymer support containing oxirane [.

Abstract: A simple amperometric biosensor for the analytical detection of hydrogen peroxide was developed. The biosensor was prepared by the immobilization of glucose oxidase (GOD) in the nanostructure matrix constructed through electrochemical deposition of Au nanoparticles (AuNPs) upon 2, 3-dimercaptosuccinic acid (DMSA) SAMs formed on Au electrode. The fabricated biosensor had a fast response of H₂O₂ (<10 s), and an excellent linear range of concentration from 2×10⁶ to 3.5×10³ M with a low detection limit of 0.65 μM (S/N = 3) under the optimum conditions. The Michaelis-Menten constant of the immobilized GOD was 2.0 mM, displaying a high affinity of the GOD toward H₂O₂ without loss of enzymatic activity in nanosubstrate.

Abstract: Objective: Making a glucose sensor to detect the glucose which is extracted from the tissue fluid on reverse iontophoresis. Method: In the role of the catalysis of glucose oxidase which was fixed in polyethylene oxide gel, the glucose and potassium ferricyanide were change into gluconic acid and potassium ferrocyanide. Then we could get the concentration of glucose by detecting the current which was created by the redox reaction. Results: The glucose sensors could detect the concentration of glucose in the range of 2.2~22mmol/l and have a good linear too. The conformance test results show that the deviation of multiple measurements of the same sensor is less than 2% and the reaction time is less than 1s. Conclusion: The sensors could detect the blood glucose.

Abstract: We report on the utilization of Pd-Pt nanodendrites (Pd-Pt NDs) to create a highly responsive glucose biosensor. Glucose oxidase was simply mixed with Pd-Pt NDs and cross-linked on the Pt electrode with chitosan (CHIT) medium by glutaraldehyde. The biosensor showed low linear detection limit (0.6 μM), wide linear detection range (0.001-5 mM). The excellent performance of the biosensor is attributed to relatively large surface areas and particularly catalytic active of Pd-Pt NDs, and good biocompatibility of CHIT, which enhances the enzyme absorption and promotes electron transfer between redox enzymes and the surface of electrodes. This composite film can be easily extended to immobilize other biomolecules.