Papers by Keyword: Glycerophosphate

Abstract: For successful reconstruction of skeletal defects, a range of materials including ceramics, polymers and their composites have been developed. The goal of our work is to prepare mineralized PCL/gelatin composite scaffolds in a double diffusion system as implants for bone tissue engineering application. Fibrous PCL/gelatin scaffold fabricated via electrospinning followed by immersing into disodium-β-glycerophosphate(β-GP) (10 mg/ml) for 12h were used as substrates for calcium phosphate (CaP) mineralization. The precipitation reaction was biomimetically carried out in a double diffusion system for a week. The CaP minerals precipitated on the scaffold were characterized by X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy and high-resolution transmission electron microscopy. The results show that apatite aggregates are combination of HAP, DCPD and ACP. β-GP can effectively promote the formation of CaP crystals. The composite scaffold fabricated in this paper hold promise for use in bone tissue engineering.

Abstract: The primary purpose of this study was to characterize the main features of a BCP-loaded chitosan-GP composite. The two-syringe design improves the storage conditions, facilitates the sterilization procedure and provides an easy-to-use injectable biomaterial, ensuring reproducible properties with minimal manipulation. Rheological measurements confirm that the chitosan- GP/BCP composite retains the thermosensitive properties already described for chitosan-GP hydrogels. At 37°C, the system gels within 10 minutes and reaches sufficient consistency after 30 minutes to prevent the mineral granules from migration into the surrounding tissues in vivo. The compressive force needed for the injection of chitosan-GP/BCP before gelation is approximately 6.6 N, only about 6 times that required for water and much lower than the average force that the majority of adults can exert. Morphology was investigated by environmental scanning electron microscopy (ESEM), which revealed 3-D dispersion of BCP granules embedded in chitosan-GP hydrogel. This open, porous structure affords complete access for body fluids and cells to each mineral granule immediately following implantation. The design using disposable syringes equipped with 16G hypodermic needles described here allows easy in vivo delivery of a fully injectable biomaterial containing porous scaffold that naturally enhances the osteogenic activity recognized for both chitosan and BCP.