Papers by Keyword: Hemolysis

Abstract: Antibiotic-resistant has emerged without new drug challenges. Polymyxin B (PMB) was the last resort therapy for multiple-drug resistant Gram-negative bacteria. However, the toxicity of PMB including nephrotoxicity (61%) and neurotoxicity (7%) was dose-limitation. PMB-based sodium deoxycholate sulfate (SDCS) formulations were prepared in the 2-different mole ratios of SDCS to PMB (5:1 and 10:1). Particle size, zeta-potential, and drug content were evaluated. The biocompatibility of PMB formulations was investigated with normal human primary renal proximal tubule epithelial cells (PCS-400-010), human kidney epithelial cell lines (HEK 293T/17), human kidney cell lines (WT 9-12), macrophage-like cells (RAW 264.7) and red blood cells (RBC). PMB formulations had smaller particle sizes and lower zeta-potential when compared to PMB. PMB content presented from 97-100% after lyophilization. PMB-SDCS formulations revealed lower toxicity to cell lines than PMB, especially SDCS: PMB (5:1) and low lysis of RBC. PMB-SDCS mixture had better biocompatibility than those PMB and SDCS alone.

Abstract: Red blood cells suspension was infused into the microchannel in micron-sized made of PDMS and this leaded to the collision between the red blood cells and the wall of the microchannel. The microflow video was observed and recorded with optical microscope and high-speed camera used. The visualizing of the movement and collision of red blood cells was achieved. The flow images were optimized and the values of the geometric parameters of the red blood cells at different positions were obtained. Combined with the experimental data, the fitted curves of the geometric parameters on the axis of the microchannel were achieved. Besides, the comparison among the flow characteristics for different flow rates was also completed. It turned out that the width of erythrocytes in the microchannel increased and the length decreased continuously along the flow direction, so did the elongation. Erythrocytes gradually developed into tabular shape from initial slender shape along the flow direction. Whats more, the trends of the geometric parameters at different flow rates showed obvious similarity.

Abstract: To evaluate the blood and tissue compatibility of the dental implant samples, the whole blood clotting time, prothrombin time and partial prothrombin time, hemolytic rate and local implantation tests of the dental implant samples were determined, which will provide the basis for the clinical safety of such medical products. The WBCT, PT and PTT results showed that there were no significant difference (p> 0.05)between the testing groups and the negative control group, hemolysis rate of the dental implant samples was 1.38%, less than 5%, which in line with medical equipment blood compatibility test, all of these results indicated that the dental implant had a good blood and tissue compatibility.

Abstract: Compared to centrifugal blood pumps, the high rotating speed of axial blood pumps lead to blood damage more easily. In order to improve blood compatibility of the axial blood pump developed by the authors, traditional method and three-dimensional streamlined method are used for axial impeller design, and rapid prototyping with ABS organic materials are adopted. Finally, hydraulic experiments and hemolysis tests have been conducted. The results reveal that the impeller design and the design parameters (diameter and length) affect the hydraulic performance and hemolysis of the axial pump obviously. The hemolysis index in axial flow impeller pump using traditional method is 0.12, while the minimum value of hemolysis index in the streamlined axial blood pump is 0.06, below the permitted hemolysis value of 0.1.

Abstract: Hydroxyapatite (HAP) and tricalcium phosphate (TCP) nanoparticles (NPs) are in current use covering a wide range of medical applications, hence, it is indispensable to study the potential side effect acting on living organism, and to figure out the biocompatibility evaluation model of nanoparticles for standardization and methodology. In the present study, HAP and TCP NPs were selected and their diameters were within 30-80nm. Cytotoxicity in vitro was analyzed by agar overlay and direct contact exposure test was conducted to evaluate potential toxicity to SD rat macrophages, and hemolysis test of NPs was performed in compliance with ISO 10993-12 guidelines. The results showed that both HAP and TCP NPs could inhibit proliferation of macrophages when their concentration was reached to 20 µg/ml, and the extent of haemolysis would be increased more than 5% while 1000µg/ml NPs were contacted with rabbit blood cells. Furthermore, TCP NPs could induce obvious hemolysis reaction and inhibit proliferation of macrophages compared with group of HAP NPs at the same concentration.