Papers by Keyword: Human-Like Collagen

Abstract: This work describes the aqueous-solution technique preparation and characterization of the complex between human-like collagen and copper (II). Pure human-like collagen and the copper complex were characterized by thermo gravimetric (TG) and differential scanning calorimetry (DSC) analysis. The TG curves and DSC data reveal the significant thermal differences between pure protein and the copper complex, demonstrating the formation of a new chemical compound, which is different from the free HLC and with more stable thermodynamic properties.

Abstract: Amino acids, the basic unit of protein molecules, are closely related to some important biological activities and they affect cell growth and metabolism directly or indirectly. Previous studies showed that adding amino acids can improve the productivity of human-like collagen (HLC). Thus, we analyzed the amino acids metabolism during the fermentation process. The results of metabolic flux analysis of amino acids implied that the cell growth and production of HLC were active as long as the free amino acids in the medium were adequate. This suggested control the concentration of amino acids could improve cell growth and human-like collagen production.

Abstract: In this paper, the cross-linking injection hydrogel were synthesized by EDC crosslinker and Carboxymethyl chitosan (CMCS)/Human-like collagen (HLC). Cytotoxicity was assessed by Methylthiazolydiphenyl-tetrazolium bromide (MTT) assay which indicated that the hydrogels was non-toxic to the BHK21 cell .Chondrocyct-encapsulation of this hydrogel were studied in order to asses the cells compatibility of the injection gel. The result showed that the material has no cytotoxicity to the cells and promoted cell adhesion and proliferation. Injected those hydrogels into mice subcutaneous , the following parameters were evaluated: inflammatory response, vascularization, new hypoderm generation. After 2,4,12,and 24 weeks of healing, the rats were sacrilifced suggested that gels in animals did not induce inflammation obvious, vessel bestrid the material after 4 weeks injection, new hypoderm generated in 12 weeks and packaged the hydrogels after 24 weeks. Consequently the gels are promised for the application in the biomaterials area.

Abstract: Recently fed-batch strategy is well known as the cheapest, simplest and more effective processes. In order to save the manual cost the automatic control strategies have been used in fermentation processes, where the addition of nutrient is added with on-line parameters monitoring. This paper mainly introduces the application of Micro Control Unit （MCU） and Back Propagation （BP）neural networks in the controlling fermentation process.

Abstract: In this paper, the cross-linking hydrogels were synthesized by EDC crosslinker and Carboxymethyl chitosan (CMCS)/Human-like collagen (HLC). The morphology of the hydrogels was observed by scanning electron microscope (SEM). The important performance of these hydrogels is biodegradation which was assessed in two ways: (1) Through enzyme solution experiment in vitro , investigating in vitro degradation property. (2) Injecting the hydrogels to mice subcutaneous , after 2,4,12,and 24 weeks of healing ,the rats were sacrificed and explanted specimens were prepared for histology analysis. Investigating degradation time in vitro and in vivo , and studying the histology .HLC hydrogels degradation time gets eight to ten months, in addition has good compatibility of the histological performances. The hydrogels are promised for the applications in the biomaterials area.

Abstract: Hydrogels were prepared from carboxymethyl chitosan (CMCS)/Human-like collagen(HLC) by S-acetylmercaptosuccinic anhydride(SAMSA) crosslinker. Fourier transform infrared (FTIR) spectroscopy indicated that there exactly existed interaction HLC, carboxymethyl chitosan and SAMSA. The morphology of the hydrogels was observed by scanning electron microscope (SEM). The equilibrium swelling ratio was also evaluated. Cell culture results showed that these hydrogels were good in promoting the cell attachment and proliferation. The hydrogels are promised for the applications in the biomaterials area.

Abstract: Protected mercaptan groups may be introduced into Human-like collagen (HLC) under very mild conditions with S-acetylmercaptosuccinic anhydride(S-AMSA) as reagent. Fluorometric assay, Fluorescence Spectra and SDS-PAGE were employed to investigate the interaction between HLC and S-AMSA. These results obtained from this study provide theoretical evidence for the further investigate.

Abstract: Two control models, manual way and automatic way, have been compared during the fermentation process in this study. The automatic way is achieved by a fermentation controller developed by ourselves. Compared to manual way, biomass (OD₆₀₀) and the production of human-like collagen (HLC) could be increases significantly, reaching to 144 and 7.12g/L respectively. Acetic acid, a key byproduct, is also lowered very obviously. Beside the production controlled by automation model was very stable, the automatic controller can regulate the feeding rate in real time and save labor force.