Papers by Keyword: Human Serum Proteins

Abstract: Hydroxyapatite (HAP) was synthesized in Triton X-100 microemulsion and was characterized by scanning electron microscopy (SEM), energy dispersive spectrometer (EDS). Particle size analysis results showed that the particles has nanometer size, high surface area and loose structure. The prepared HAP had high adsorption capacity to human serum protein and the adsorption of human serum protein on the surface of HAP was confirmed by SEM. The factors which influenced the adsorption capacity such as the acidity, reaction time and the concentration of adsorbent were investigated in detail and the adsorption isothermal was also obtained. The adsorption capacity reduced while the pH value increased, and the adsorption reached equilibrium when shaking time was up to 40 min. The adsorption isotherm could be fitted by the pseudo-Langmuir type. The adsorption of human serum albumin on HAP was investigated, and the influences of adsorption on the conformation of human serum albumin were studied by infrared and ultraviolet spectroscopy. It can be seen from the FT-IR spectra that the intensity of flex vibration of C=O, N-C bonds all weakened. The calculation results of ultraviolet spectrum indicated that the quantity of α spiral structure reduced from 44% to 17.12%.

Abstract: In this paper, the adsorption of human serum albumin (HSA), human serum fibrinogen (HFG) and human serum immune globulin (IG) on surfaces of diamond like carbon film (DLC), diamond film (DF) and graphite has been studied. The adsorption isotherms of single component protein solution and the competitive adsorption of binary system have been investigated by radioisotope 125 I labeling method. Results showed that (1) the adsorptive amounts of HSA on DLC is more than that of HFG, but the adsorptive amounts of HFG on DF and graphite are apparently more than those HSA; (2) the relative competitive adsorption ability of three proteins on DF and graphite surfaces is HFG > IG > HSA, but that on DLC is HFG ≈ HAS > IG, comparison with HSA, there is no apparent competitive adsorption superiority on DLC for HFG. These results indicated that there is no apparent distinction for the adsorption of three human serum proteins on DLC, but the adsorption of HFG on DF and graphite takes precedence in varying degrees. It probably makes rational explanation for the result of blood-compatibility tests in vitro that DLC is good, but DF and graphite are worse.

Abstract: The adhesion of bovine collagen type I, bovine serum albumin, bovine IgG, 1 % and 10 % (v/v) human serum to hydroxyapatite (HA), silicon-substituted hydroxyapatite (Si-HA) and tissue culture plastic were studied. The materials were incubated at 37 °C for 30 minutes, after which the protein solution was removed and analyzed. The adsorbed protein was evaluated by electrophoresis and immunoassay after extraction from the materials. The degree of adhesion was higher for collagen, followed by IgG and albumin on all materials. However there was no difference in the amount of collagen adsorbed onto the surface of each material and this was also the finding with albumin and IgG. These results suggest that the increased bioactivity seen with Si-HA is not due to the degree of protein adhesion, but may possibly be due to changes in the conformation of the bound proteins.