Papers by Keyword: In Vitro Biocompatibility

Abstract: Studies about silicon-substituted hydroxyapatites exhibit several shortcomings that leave unanswered questions regarding the properties and subsequent biological outcomes generated by this biomaterial. Firstly, samples characterization is often incomplete, meaning that phase purity on the pellet surface is not assured. In fact, ceramic materials used in literature that are claimed to be pure are actually polluted through second phase as superficial polymerized silicate. In this study, we have successfully synthesized a phase pure silicon hydroxyapatite powder Ca₁₀(PO₄)_5.5(SiO₄)_0.5(OH)_1.5 (Si_0.5HA) compressed this powder into pellets, sintered them, and evaluated the biological response of osteoblast cells (C3H10 line) seeded on the pellet surface. Besides, the solubility in aqueous media of HA and Si_0.5HA pellets were determined through static experiments. These tests attempt to provide a comprehensive picture of the cellular response to the SiHA material, in order to determine the mechanism by which Si evokes the improved in vitro biological outcomes described in the literature. Results revealed first an equivalent solubility of Si_0.5HA and HA pellets, and second that cells do not react favourably to the pure SiHA surface.

Abstract: The in vitro biocompatibility of aragonite material obtained from inner and out layers of four different molluscs was tested. After grinding and sieving, the obtained fine powders were put in contact with primary osteoblasts derived from rat calvariae. The viability of the cells increased at about 10% in the presence of powders derived from Vennus Gallina outer layer and from Pecten Jacobaeus inner layer. In the case of the presence of the other 6 tested powders, there was no statistical difference in cells’ viability. With regard to alkaline phosphatase production, all the tested powders induced a decrease of the production of this enzyme by osteoblasts. There was no evidence of any alterations in collagen production.