Papers by Keyword: Lindane

Abstract: Lindane (γ-hexachlorocyclohexane) was used as the substrate for a degradation experiment with the white rot fungi Phlebia brevispora TMIC34596 and Phlebia lindtneri GB1027, which are capable of degrading DDT. Pure culture of both fungi showed that about 40% of lindane was degraded after 7 days of incubation, while over 70% of lindane was degraded after 28 days of incubation. Eight metabolic products such as pentachlorocyclohexanol, dihydroxytetrachlorocyclohexane and trihydroxytrichlorocyclohexane were detected from both fungal cultures using gas chromatography/mass spectrometry (GC/MS). This is the first report of the biodegradation of lindane through successive Cl/OH substitution pathway by microorganisms.

Abstract: In order to investigate the capability of degradation lindane and lignocellulose of a microbial community, the gas chromatography(GC) was used to determine the concentration of lindane. After 18 days cultured, lindane reduced 6.25% more than the control. And the result of gas chromatography and mass spectrometry (GCMS) showed that the species and quantum of fermentation productions changed much in different phase. In order to determine the bacterial composition of the community, clone library was used to clarified bacterial composition. Constructed 16S rDNA clone library showed 60% closest relative among them were known the detailed information. Strain of closest relative of Clostridium may be main contribute to lignocellulose degradation, and closest relative of Proteobacterium may be the main contribute to degradation organochlorine pesticide (lindane).