Papers by Keyword: Lipid Accumulation

Abstract: Effects of different carbon source and Fe³⁺ for the growth and lipid accumulation of Monoraphidium sp. FXY-10 cultured mixotrophically was studied in our present work. The final cell density was reached to 2.626 g L^-1 when glucose was the only carbon source, which is 1.43-fold to sodium acetate (1.834 g L^-1), far higher than sucrose (0.251 g L^-1) and xylitol (0.471 g L^-1), but barely grow in other culture condition. Additionally, the highest algae lipid productivity (77.45 mg L^-1 d^-1) was obtained in 10 g L^-1 glucose group, which indicated that glucose was the optimal carbon source for growth and lipid accumulation of Monoraphidium sp. FXY-10. Nevertheless, Monoraphidium sp. FXY-10 was grew slowly in BG-11 culture medium with the absence of Fe³⁺. The biomass was achieved at the top with 50μM Fe³⁺ added. With the increase of Fe³⁺ concentration, it showed no variation in the growth of microalgae. The highest biomass productivity (209.87 mg L^-1 d^-1) was reached when the Fe³⁺ concentration was at 150μM while highest lipid productivity (94.05 mg L^-1 d^-1) reached at 50μM, which indicated that Fe³⁺ was one of the most indispensable trace elements for the growth and lipid accumulation of Monoraphidium sp. FXY-10.

Abstract: The purpose of this study is to make use of trans10,cis12 CLA (t-CLA) that has potential for proliferation and differentiation to form adipocyte on the collagen-coated surface. Results provided evidences of good adhesion, growth, viability, and differentiation of adipocyte on collagen-coated surface compared with non-coated surface. Also, the results showed that mouse 3T3-L1 preadipocyte can be successfully and reproducibly cultured on the collagen-coated surface, and the adipocyte precursor cells placed on the collagen-coated surface are able to undergo full maturation into adipocytes in the control cells. Moreover, glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 preadipocyte cultured on collagen-coated surface with t-CLA was higher than that on polystyrene (PS) surface due to higher cell adhesion and cell viability. These results suggest that collagen coating may provide a promising approach to develop a new adipocyte replacement strategy using CLA.